Somatostatin Therapy Improves Stellate Cell Activation and Early Fibrogenesis in a Preclinical Model of Extended Major Hepatectomy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Study Overview
- -
- 75% hepatectomy (75H): left trisectionectomy and follow-up for 24 h (N = 6) and 5 days (N = 6);
- -
- 90% hepatectomy (90H): left trisectionectomy + right lateral sectionectomy and follow-up for 24 h (N = 6) and 5 days (N = 6);
- -
- 90% hepatectomy + SST (90H + SST): left trisectionectomy + right lateral sectionectomy + continuous perioperative infusion of SST and follow-up for 24 h (N = 6) and 5 days (N = 6).
2.3. Preoperative Care, Anesthesia, and Monitoring
2.4. Surgical Procedure
2.5. Postoperative Follow-Up
2.6. Serum Biochemical Analyses
2.7. Tissue Analyses
2.8. Hepatocellular Proliferation and Apoptosis
2.9. Hepatic Stellate Cell Activation, Collagen Deposition, and Changes in the Extracellular Matrix
2.10. Liver Sinusoidal Endothelial Cell Injury
2.11. Data and Statistical Analyses
3. Results
3.1. Hepatic Hemodynamic Parameters
3.2. Hepatocellular Injury
3.3. Hepatic Regeneration
3.4. Hepatocellular Apoptosis
3.5. Extracellular Matrix Remodeling, Hepatic Stellate Cell Activation, and Collagen Deposition
3.6. Liver Sinusoidal Endothelial Cell Injury
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | Time Point | 75H | 90H | 90H + SST | p |
---|---|---|---|---|---|
PVF (mL/min) | Baseline | 778 (600–950) | 833 (600–1100) | 910 (650–1010) | 0.16 |
- | End surgery | 830 (600–1075) | 866 (630–1120) | 739 (525–1070) | 0.12 |
- | 24 h | 938 (750–1100) | 881 (700–1050) | 868 (700–1050) | 0.52 |
- | 5 days | 955 (730–1160) | 803 (600–900) | 830 (600–1000) | 0.60 |
- | Baseline | 3 1–4 | 3 1–5 | 3 1–5 | 0.90 |
PVPG (mmHg) | End surgery | 4 2–6 | 8 3–13 | 4 2–6 | <0.001 |
- | 24 h | 3 2–4 | 4 3–6 | 4 2–6 | 0.52 |
- | 5 days | 5 4–7 | 7 6,7 | 5 2–6 | 0.20 |
HAF (mL/min) | Baseline | 140 80–190 | 147 100–190 | 144 90–175 | 0.78 |
- | End surgery | 63 30–90 | 57 25–100 | 77 30–120 | 0.08 |
- | 24 h | 71 50–90 | 57 30–90 | 65 30–90 | 0.57 |
- | 5 days | 78 50–115 | 57 38–75 | 82 35–140 | 0.45 |
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Hessheimer, A.J.; Vengohechea, J.; Martínez de la Maza, L.; Muñoz, J.; Vendrell, M.; Sanahuja, J.M.; Torroella, A.; Adel Al Shwely, F.; Riquelme, F.; Muñoz, C.; et al. Somatostatin Therapy Improves Stellate Cell Activation and Early Fibrogenesis in a Preclinical Model of Extended Major Hepatectomy. Cancers 2021, 13, 3989. https://doi.org/10.3390/cancers13163989
Hessheimer AJ, Vengohechea J, Martínez de la Maza L, Muñoz J, Vendrell M, Sanahuja JM, Torroella A, Adel Al Shwely F, Riquelme F, Muñoz C, et al. Somatostatin Therapy Improves Stellate Cell Activation and Early Fibrogenesis in a Preclinical Model of Extended Major Hepatectomy. Cancers. 2021; 13(16):3989. https://doi.org/10.3390/cancers13163989
Chicago/Turabian StyleHessheimer, Amelia J., Jordi Vengohechea, Lilia Martínez de la Maza, Javier Muñoz, Marina Vendrell, Josep Martí Sanahuja, Alba Torroella, Farah Adel Al Shwely, Francisco Riquelme, César Muñoz, and et al. 2021. "Somatostatin Therapy Improves Stellate Cell Activation and Early Fibrogenesis in a Preclinical Model of Extended Major Hepatectomy" Cancers 13, no. 16: 3989. https://doi.org/10.3390/cancers13163989