Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER–Mitochondria Interface in Oncogene-Driven NSCLC
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Silencing of PDK1 by Targeted shRNA and Effects on Glucose Metabolism
2.2. Effects of PDK1 Knockdown on Apoptotic Response to TKI Treatment
2.3. PDK1-Dependent Protein–Protein Interactions at ER–Mitochondria Interface
2.4. Effects of PDK1 Knockdown on Tumor Growth in Animal Models
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Treatment
4.2. shRNA and siRNA Interference
4.3. Immunoblotting Analysis and Immunoprecipitation
4.4. Glucose Consumption, Intracellular ATP and Citrate Levels in Cultured Tumor Cells
4.5. Oxygen Consumption and Extracellular Acidification Rates
4.6. PDK1 Knockdown and Tumor Growth in Animal Models
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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De Rosa, V.; Iommelli, F.; Terlizzi, C.; Leggiero, E.; Camerlingo, R.; Altobelli, G.G.; Fonti, R.; Pastore, L.; Del Vecchio, S. Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER–Mitochondria Interface in Oncogene-Driven NSCLC. Cancers 2021, 13, 4133. https://doi.org/10.3390/cancers13164133
De Rosa V, Iommelli F, Terlizzi C, Leggiero E, Camerlingo R, Altobelli GG, Fonti R, Pastore L, Del Vecchio S. Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER–Mitochondria Interface in Oncogene-Driven NSCLC. Cancers. 2021; 13(16):4133. https://doi.org/10.3390/cancers13164133
Chicago/Turabian StyleDe Rosa, Viviana, Francesca Iommelli, Cristina Terlizzi, Eleonora Leggiero, Rosa Camerlingo, Giovanna G. Altobelli, Rosa Fonti, Lucio Pastore, and Silvana Del Vecchio. 2021. "Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER–Mitochondria Interface in Oncogene-Driven NSCLC" Cancers 13, no. 16: 4133. https://doi.org/10.3390/cancers13164133
APA StyleDe Rosa, V., Iommelli, F., Terlizzi, C., Leggiero, E., Camerlingo, R., Altobelli, G. G., Fonti, R., Pastore, L., & Del Vecchio, S. (2021). Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER–Mitochondria Interface in Oncogene-Driven NSCLC. Cancers, 13(16), 4133. https://doi.org/10.3390/cancers13164133