The Effect of a Histone Deacetylase Inhibitor (AR-42) and Zoledronic Acid on Adult T-Cell Leukemia/Lymphoma Osteolytic Bone Tumors
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cells and Reagents
2.2. Transduction of MT-2 Cells with Luciferase
2.3. Bioluminescent Imaging
2.4. Cell Viability Assays
2.5. Treatment Study
2.6. Histopathology
2.7. Radiographic Imaging
2.8. Microcomputed Tomography (μCT)
2.9. Real-Time Reverse Transcription-PCR (qRT-PCR)
2.10. Statistical Analysis
3. Results
3.1. MTT Viability Assays
3.2. Development of MT-2 Intratibial Mouse Model
3.3. Bioluminescence, Tumor Growth and Tibial Tumor Burden
3.4. Faxitron Radiography and Analysis
3.5. Histopathology of Intratibial Tumors
3.6. qRT-PCR
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Forward Primers | Reverse Primers |
---|---|---|
GAPDH | GCAAATTCCATGGCACCGTC | AGCATCGCCCCACTTGATTT |
PTHrP | GTCTCAGCCGCCGCCTCAA | GGAAGAATCGTCGCCGTAAA |
MIP-1α | CTGCATCACTTGCTGCTGACA | CACTGGCTGCTCGTCTCAAAG |
ENPP2 (autotaxin) | GGAACGCAGATGGCATGTTG | TTATCAAATCCGTGGTCTCCCT |
Wnt5a | CTACGAGAGTGCTCGCATCC | GCCAGGTTGTACACCGTCC |
Interleukin-6 (IL-6) | GAGGAGACTTGCCTGGTGAAA | TGGCATTTGTGGTTGGGTCA |
CXCR4 | CGGTTACCATGGAGGGGATCA | ATGACCAATCCATTGCCCACA |
TAX | CCGCCGATCCCAAAGAAA | CCGAACATAGTCCCCCAGA |
HBZ | AACTTACCTAGACGGCGGAC | CATGGCACAGGCAAGCATCG |
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Elshafae, S.M.; Kohart, N.A.; Breitbach, J.T.; Hildreth, B.E., III; Rosol, T.J. The Effect of a Histone Deacetylase Inhibitor (AR-42) and Zoledronic Acid on Adult T-Cell Leukemia/Lymphoma Osteolytic Bone Tumors. Cancers 2021, 13, 5066. https://doi.org/10.3390/cancers13205066
Elshafae SM, Kohart NA, Breitbach JT, Hildreth BE III, Rosol TJ. The Effect of a Histone Deacetylase Inhibitor (AR-42) and Zoledronic Acid on Adult T-Cell Leukemia/Lymphoma Osteolytic Bone Tumors. Cancers. 2021; 13(20):5066. https://doi.org/10.3390/cancers13205066
Chicago/Turabian StyleElshafae, Said M., Nicole A. Kohart, Justin T. Breitbach, Blake E. Hildreth, III, and Thomas J. Rosol. 2021. "The Effect of a Histone Deacetylase Inhibitor (AR-42) and Zoledronic Acid on Adult T-Cell Leukemia/Lymphoma Osteolytic Bone Tumors" Cancers 13, no. 20: 5066. https://doi.org/10.3390/cancers13205066