Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Material Preparation of Gold-Collagen-Berberine (Au-Col-BB)
2.2. Material Characterization
2.3. Cell Culture
2.4. Examination of Cell Viability
2.5. Cellular Uptake Assay
2.6. LysoTracker Assay
2.7. Cell Cycle Analysis
2.8. Annexin V-PI Staining Assay
2.9. Gelatin Zymography Analysis
2.10. Assessment of Cell Migration Ability
2.11. Western Blot Assay of Apoptotic Protein Expression
2.12. Tumor Xenograft Mouse Model
2.13. Statistics Analysis
3. Results
3.1. Material Characterization of the AuNP-Collagen-Berberine (Au-Col-BB) Nanocarriers
3.2. Assessment of Au-Col-BB on Cell Viability and Cytotoxicity on A549, Colo-205, and Her-2 Cell Lines
3.3. Investigation of Biocompatibility in BAEC and Her-2 Cell Line Treating with Various Materials
3.4. Investigation of Cell Uptake Efficiency between Her-2 and BAEC Cell Lines
3.5. Assessment of Potential Cellular Transportation between Her-2 and BAEC Cell Lines
3.6. Assessment of Migratory Inhibition Effects
3.7. Assessment of Apoptotic Related Proteins Expression
3.8. Investigation of Tumor Suppression Effects
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chiu, C.-F.; Fu, R.-H.; Hsu, S.-h.; Yu, Y.-H.; Yang, S.-F.; Tsao, T.C.-Y.; Chang, K.-B.; Yeh, C.-A.; Tang, C.-M.; Huang, S.-C.; et al. Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer. Cancers 2021, 13, 5317. https://doi.org/10.3390/cancers13215317
Chiu C-F, Fu R-H, Hsu S-h, Yu Y-H, Yang S-F, Tsao TC-Y, Chang K-B, Yeh C-A, Tang C-M, Huang S-C, et al. Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer. Cancers. 2021; 13(21):5317. https://doi.org/10.3390/cancers13215317
Chicago/Turabian StyleChiu, Chen-Feng, Ru-Huei Fu, Shan-hui Hsu, Yang-Hao (Alex) Yu, Shun-Fa Yang, Thomas Chang-Yao Tsao, Kai-Bo Chang, Chun-An Yeh, Cheng-Ming Tang, Sheng-Chu Huang, and et al. 2021. "Delivery Capacity and Anticancer Ability of the Berberine-Loaded Gold Nanoparticles to Promote the Apoptosis Effect in Breast Cancer" Cancers 13, no. 21: 5317. https://doi.org/10.3390/cancers13215317