MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. GSC-Lines and Cell Culture
2.2. Patient Cohort
2.3. Transfection
2.4. Cell Viability Assay
2.5. Pyrosequencing and Methylation
2.6. Subcellular Fractionation and Western Blotting
2.7. Immunofluorescence
2.8. Cell Cycle Analysis
2.9. Transcriptome Analysis
2.10. Statistical Analysis
3. Results
3.1. Nuclear and Heterogeneous MEOX2 Expression among GSC
3.2. MEOX2 Expression Is Partly Dependent on the Methylation Status of Its Promoter
3.3. MEOX2 Is Involved in Cell Survival through ERK/MAPK and PI3K/AKT Pathways
3.4. The Loss of MEOX2 Expression Correlates with the Differentiation of GSC and with Acquisition of Neuronal Lineage Features
3.5. Inhibition of MEOX2 Regulates a Restricted Gene Network including Cadherin CDH10
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tachon, G.; Masliantsev, K.; Rivet, P.; Desette, A.; Milin, S.; Gueret, E.; Wager, M.; Karayan-Tapon, L.; Guichet, P.-O. MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells. Cancers 2021, 13, 5943. https://doi.org/10.3390/cancers13235943
Tachon G, Masliantsev K, Rivet P, Desette A, Milin S, Gueret E, Wager M, Karayan-Tapon L, Guichet P-O. MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells. Cancers. 2021; 13(23):5943. https://doi.org/10.3390/cancers13235943
Chicago/Turabian StyleTachon, Gaëlle, Konstantin Masliantsev, Pierre Rivet, Amandine Desette, Serge Milin, Elise Gueret, Michel Wager, Lucie Karayan-Tapon, and Pierre-Olivier Guichet. 2021. "MEOX2 Transcription Factor Is Involved in Survival and Adhesion of Glioma Stem-like Cells" Cancers 13, no. 23: 5943. https://doi.org/10.3390/cancers13235943