Modeling Tumor: Lymphatic Interactions in Lymphatic Metastasis of Triple Negative Breast Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
1.1. Lymphatic Metastasis of Breast Cancer (BC)
1.2. Lymphatic Metastasis of Triple Negative Breast Cancer (TNBC)
1.3. Avatars for Studying Lymphatic Metastasis
2. Materials and Methods
2.1. Reagents
2.2. Cells and Cell Maintenance
2.3. 3D Culture
2.4. Image Acquisition for Quantitative Analysis in 3D
2.5. LEC-Conditioned Media (CM)
2.6. 3D Invasion Assays
2.7. Fabrication of TAME Chambers
2.8. Statistical Analysis
3. Results
3.1. TNBC:LEC Interactions in 3D Cocultures
3.2. LEC Secretome and TNBC Invasion
3.3. Tissue Architecture and Microenvironment Engineering (TAME) Chambers
3.4. LEC Secretome-Induced TNBC Invasion in TAME Linked-Well Chambers
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ji, K.; Zhao, Z.; Sameni, M.; Moin, K.; Xu, Y.; Gillies, R.J.; Sloane, B.F.; Mattingly, R.R. Modeling Tumor: Lymphatic Interactions in Lymphatic Metastasis of Triple Negative Breast Cancer. Cancers 2021, 13, 6044. https://doi.org/10.3390/cancers13236044
Ji K, Zhao Z, Sameni M, Moin K, Xu Y, Gillies RJ, Sloane BF, Mattingly RR. Modeling Tumor: Lymphatic Interactions in Lymphatic Metastasis of Triple Negative Breast Cancer. Cancers. 2021; 13(23):6044. https://doi.org/10.3390/cancers13236044
Chicago/Turabian StyleJi, Kyungmin, Zhiguo Zhao, Mansoureh Sameni, Kamiar Moin, Yong Xu, Robert J. Gillies, Bonnie F. Sloane, and Raymond R. Mattingly. 2021. "Modeling Tumor: Lymphatic Interactions in Lymphatic Metastasis of Triple Negative Breast Cancer" Cancers 13, no. 23: 6044. https://doi.org/10.3390/cancers13236044