IFI35 Promotes Renal Cancer Progression by Inhibiting pSTAT1/pSTAT6-Dependent Autophagy
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patient Specimens and Ethics Statement
2.2. Immunohistochemistry (IHC) Staining
2.3. Western Blotting
2.4. Cell Culture and Stable Cell Line Selection
2.5. Cell Proliferation Assay
2.6. Cell Apoptosis Analysis
2.7. Cell Invasion Assay
2.8. Analysis of Autophagy by Fluorescence Microscope
2.9. Flow Cytometry Analysis
2.10. Animal Models
2.11. Pathological Analysis
2.12. Statistical Analysis
3. Results
3.1. Increased Expression of IFI35 Is Correlated with Poor Prognosis in RCC
3.2. Knockdown of IFI35 Inhibits Cell Proliferation and Invasion and Promotes Cell Apoptosis in Renal Cancer Cells
3.3. IFI35 Knockdown Suppresses the Tumor Growth or Metastasis in Subcutaneous or Lung Metastasis Models of Renal Cancer
3.4. Knockdown of IFI35 Enhances pSTAT1/pSTAT6 Dependent Autophagy in Renal Cancer Cells
3.5. Autophagy Induced by the Knockdown of IFI35 Is Critical for Inhibiting the Malignant Behavior of Renal Cancer Cells and Tumor Growth
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variables | IFI35 Staining | |||
---|---|---|---|---|
Low (%) | High (%) | Total | p * | |
Age | ||||
>56 years | 40 (42.4) | 68 (57.6) | 113 | 0.761 |
≤56 years | 36 (47.3) | 56 (52.7) | 87 | |
Gender | ||||
Male | 52 (56.7) | 80 (43.1) | 132 | 0.571 |
Female | 24 (54.3) | 44 (45.7) | 68 | |
Tumor size | ||||
≤7 cm | 56 (54.6) | 98 (45.4) | 154 | 0.383 |
>7 cm | 20 (53.8) | 26 (46.2) | 46 | |
Histology Grade | ||||
I–III | 67 (72.2) | 114 (27.8) | 181 | 0.376 |
IV | 9 (54.2) | 10 (45.8) | 19 | |
pT status | ||||
pT1–pT3 | 67 (62.1) | 103 (37.9) | 187 | 0.677 |
pT4 | 4 (33.3) | 8 (66.7) | 13 | |
pN status | ||||
N0 | 63 (57.0) | 109 (43.0) | 172 | 0.639 |
N1–N3 | 8 (54.2) | 11 (45.8) | 19 | |
pM status | ||||
M0 | 68 (51.8) | 98 (49.2) | 166 | 0.135 |
M | 2 (66.7) | 9 (33.3) | 9 | |
TNM stage | ||||
I–III | 54 (59.6) | 76 (41.4) | 160 | <0.05 |
IV | 1 (75.0) | 10 (25.0) | 9 |
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Chai, D.; Shi, S.Y.; Sobhani, N.; Ding, J.; Zhang, Z.; Jiang, N.; Wang, G.; Li, M.; Li, H.; Zheng, J.; et al. IFI35 Promotes Renal Cancer Progression by Inhibiting pSTAT1/pSTAT6-Dependent Autophagy. Cancers 2022, 14, 2861. https://doi.org/10.3390/cancers14122861
Chai D, Shi SY, Sobhani N, Ding J, Zhang Z, Jiang N, Wang G, Li M, Li H, Zheng J, et al. IFI35 Promotes Renal Cancer Progression by Inhibiting pSTAT1/pSTAT6-Dependent Autophagy. Cancers. 2022; 14(12):2861. https://doi.org/10.3390/cancers14122861
Chicago/Turabian StyleChai, Dafei, Shang Yuchen Shi, Navid Sobhani, Jiage Ding, Zichun Zhang, Nan Jiang, Gang Wang, Minle Li, Hailong Li, Junnian Zheng, and et al. 2022. "IFI35 Promotes Renal Cancer Progression by Inhibiting pSTAT1/pSTAT6-Dependent Autophagy" Cancers 14, no. 12: 2861. https://doi.org/10.3390/cancers14122861