Recurrent NOMO1 Gene Deletion Is a Potential Clinical Marker in Early-Onset Colorectal Cancer and Is Involved in the Regulation of Cell Migration
, Abel Martel-Martel 1,2,4,†, Paula GarcÃa-Vallés 1,2, Luis A. Corchete 3,5,6, Juan L. GarcÃa 1,3, Nerea Gestoso-Uzal 1,2, Rosario Vidal-Tocino 1,4, Óscar Blanco 1,7, LucÃa Méndez 1,8, Manuel Sánchez-MartÃn 1,8, Manuel Fuentes 9,10, Ana B. Herrero 1,2,3, Andreana N. Holowatyj 11, José Perea 1 and Rogelio González-Sarmiento 1,2,3,*
Ryou Seishima
Round 1
Reviewer 1 Report
This is an interesting study in the new area of Molecular tumor biology leading to more research for clinical evaluation.
Author Response
Point 1: This is an interesting study in the new area of Molecular tumor biology leading to more research for clinical evaluation.
Response 1: We thank the Reviewer for this comment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
Authors has revealed that NOMO1, a gene that is specifically deleted or inactivated in EOCRCs, contributes to cancer cell migration. The manuscript was informative and persuasive. I have few minor comments:
1. Does the title accurately describe the manuscript? The more mechanistic aspect of NOMO1 is described than just a 'clinical marker'.
2. Can Fig.5 Western blot analysis results be quantified? The upregulation of HMGA1 in HS-5 is clear but that in HCT-116 is not so clear.
Author Response
Authors has revealed that NOMO1, a gene that is specifically deleted or inactivated in EOCRCs, contributes to cancer cell migration. The manuscript was informative and persuasive. I have few minor comments:
Point 1: Does the title accurately describe the manuscript? The more mechanistic aspect of NOMO1 is described than just a 'clinical marker'.
Response 1: We appreciate this suggestion from the Reviewer. The manuscript tittle has been changed to “Recurrent NOMO1 Gene Deletion is a Potential Clinical Marker in Early-Onset Colorectal Cancer and is involved in the regulation of cell migration”.
Point 2: Can Fig.5 Western blot analysis results be quantified? The upregulation of HMGA1 in HS-5 is clear but that in HCT-116 is not so clear.
Response 2: We thank the Reviewer for raising this point. Fig.5 western blot analysis has been quantified and added to the main text as Fig 5.B.
Author Response File: Author Response.docx
