A CAF-Fueled TIMP-1/CD63/ITGB1/STAT3 Feedback Loop Promotes Migration and Growth of Breast Cancer Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Lines/Sublines and Reagents
2.2. Western Blot Analysis
2.3. RNA Interference
2.4. Growth Assays
2.5. Migration Assays
2.6. Statistical Analyses
3. Results
3.1. Exposure of Breast Cancer Cells to High Concentrations of Extracellular TIMP-1 Modulates the Levels of Plasma Membrane Proteins Linked to TIMP-1 Function
3.2. TIMP-1 Is Responsible for the CAF-CM-Induced Upregulation of ITGB1
3.3. CAF-CM Activates STAT3 and ERK1/2 by Stimulating the TIMP-1/CD63/ITGB1 Complex
3.4. CAF-CM Stimulates AnD5 Cells to Secret More TIMP-1 by Activating STAT3
3.5. Knockdown of Any Component of the TIMP-1/CD63/ITGB1/STAT3 Pathway Affects Growth and Migration of AnD5 Cells
3.6. CAF-CM Stimulates TIMP-1 Secretion also in Other BC Lines
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dittmer, A.; Dittmer, J. A CAF-Fueled TIMP-1/CD63/ITGB1/STAT3 Feedback Loop Promotes Migration and Growth of Breast Cancer Cells. Cancers 2022, 14, 4983. https://doi.org/10.3390/cancers14204983
Dittmer A, Dittmer J. A CAF-Fueled TIMP-1/CD63/ITGB1/STAT3 Feedback Loop Promotes Migration and Growth of Breast Cancer Cells. Cancers. 2022; 14(20):4983. https://doi.org/10.3390/cancers14204983
Chicago/Turabian StyleDittmer, Angela, and Jürgen Dittmer. 2022. "A CAF-Fueled TIMP-1/CD63/ITGB1/STAT3 Feedback Loop Promotes Migration and Growth of Breast Cancer Cells" Cancers 14, no. 20: 4983. https://doi.org/10.3390/cancers14204983