Cancer Cells Promote Phenotypic Alterations in Hepatocytes at the Edge of Cancer Cell Nests to Facilitate Vessel Co-Option Establishment in Colorectal Cancer Liver Metastases
Abstract
:Simple Summary
Abstract
1. Introduction
2. Methods
2.1. Patient Samples
2.2. Cells Culturing and Treatment
2.3. Lentiviral shRNA Knockdown
2.4. Immunoblotting
2.5. Immunohistochemical Staining
2.6. Immunofluorescence Staining
2.7. Scratch Assay
2.8. MTT Assay
2.9. Statistical Analysis
3. Results
3.1. Upregulation Pression of Proapoptotic Biomarker Vessel Co-Opting Lesions
3.2. Cancer Cells Stimulate Apoptosis in Hepatocytes In Vitro
3.3. Cancer Cells Induce EMT and Motility in Hepatocytes
3.4. RUNX1 Is a Key Player in the Interactions between Cancer Cells and Hepatocytes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rada, M.; Tsamchoe, M.; Kapelanski-Lamoureux, A.; Hassan, N.; Bloom, J.; Petrillo, S.; Kim, D.H.; Lazaris, A.; Metrakos, P. Cancer Cells Promote Phenotypic Alterations in Hepatocytes at the Edge of Cancer Cell Nests to Facilitate Vessel Co-Option Establishment in Colorectal Cancer Liver Metastases. Cancers 2022, 14, 1318. https://doi.org/10.3390/cancers14051318
Rada M, Tsamchoe M, Kapelanski-Lamoureux A, Hassan N, Bloom J, Petrillo S, Kim DH, Lazaris A, Metrakos P. Cancer Cells Promote Phenotypic Alterations in Hepatocytes at the Edge of Cancer Cell Nests to Facilitate Vessel Co-Option Establishment in Colorectal Cancer Liver Metastases. Cancers. 2022; 14(5):1318. https://doi.org/10.3390/cancers14051318
Chicago/Turabian StyleRada, Miran, Migmar Tsamchoe, Audrey Kapelanski-Lamoureux, Nour Hassan, Jessica Bloom, Stephanie Petrillo, Diane H. Kim, Anthoula Lazaris, and Peter Metrakos. 2022. "Cancer Cells Promote Phenotypic Alterations in Hepatocytes at the Edge of Cancer Cell Nests to Facilitate Vessel Co-Option Establishment in Colorectal Cancer Liver Metastases" Cancers 14, no. 5: 1318. https://doi.org/10.3390/cancers14051318