Analysis of the Peritumoral Tissue Unveils Cellular Changes Associated with a High Risk of Recurrence
, Agnès Neuville 2, Jessica Baud 1, Gaëlle Perot 4,5, Iris Bonomo 2, Mathilde Maire 2, Maxime Michot 2, Jean-Michel Coindre 2,3, François Le Loarer 1,2,3 and Frédéric Chibon 4,5
Round 1
Reviewer 1 Report
Comments for authors and editorial teams:
The study entitled "Analysis of the peritumoral tissue unveils cellular changes associated with a high risk of recurrence” is novel and interesting. However, some minor concerns should be considered to improve the novelty as well as the flow of the manuscript:
1- Abstract: Acceptable
2- Keywords: Acceptable
3- Introduction: Acceptable but could be better by wider literature review particularly related new & update articles from domestic researchers.
4- Methodology: Acceptable
5- Results: Acceptable
6- Discussion: Could be scientifically than present level using more and wider literature review and explanation of related and domestic survey and of course then using from them as Bibliography/References.
7- Bibliography/References: More domestic and update Ref(s). are necessary. Authors should do more literature review for using related domestic articles and therefore some more domestic Ref(s). (using from domestic Ref(s). is scientific discipline for publication of academic data.)
8- I could not see running title in manuscript. It is compulsory.
9- Table & Graph quality: Acceptable
Final decision: Manuscript is acceptable for publication after minor correction
Important: Improve the quality this nice article by using new references like:
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Author Response
The study entitled "Analysis of the peritumoral tissue unveils cellular changes associated with a high risk of recurrence” is novel and interesting. However, some minor concerns should be considered to improve the novelty as well as the flow of the manuscript:
1- Abstract: Acceptable
2- Keywords: Acceptable
3- Introduction: Acceptable but could be better by wider literature review particularly related new & update articles from domestic researchers.
We would like to thank the reviewer and we modified the introduction with adding more references.
4- Methodology: Acceptable
5- Results: Acceptable
6- Discussion: Could be scientifically than present level using more and wider literature review and explanation of related and domestic survey and of course then using from them as Bibliography/References.
It has been done accordingly to the proposed references.
7- Bibliography/References: More domestic and update Ref(s). are necessary. Authors should do more literature review for using related domestic articles and therefore some more domestic Ref(s). (using from domestic Ref(s). is scientific discipline for publication of academic data.)
We added more references.
8- I could not see running title in manuscript. It is compulsory.
9- Table & Graph quality: Acceptable
We propose a running title as “Impact of peritumoral tissue in soft-tissue-sarcoma progression" if necessary.
Reviewer 2 Report
Τhe title reflects the study. This manuscript presents a clear and clinically useful message. It is well written in terms of clarity, style, and use of English language. Materials and methods approach are sufficiently detailed. The discussion section explains adequately the subject in the context of published information. The conclusions accurately and clearly explain the main clinical message. The length is ideal. All figures are of good quality and relevant to the clinical message. All references are appropriate and current.
Author Response
Reviewer 2:
Τhe title reflects the study. This manuscript presents a clear and clinically useful message. It is well written in terms of clarity, style, and use of English language. Materials and methods approach are sufficiently detailed. The discussion section explains adequately the subject in the context of published information. The conclusions accurately and clearly explain the main clinical message. The length is ideal. All figures are of good quality and relevant to the clinical message. All references are appropriate and current.
We would like to thank the reviewer for this consideration.
Reviewer 3 Report
An interesting study to show that genomic and transcriptomic pseudocapsules of prospective sarcomas were analyzed and revealed to be correlated with a higher risk of recurrence after surgery. Some point should be noted as below.
1) The title should be better as “Analysis of the peritumoral tissue unveils cellular changes associated with a high risk of recurrence in soft-tissue sarcoma”.
2) Instead of Figure 1 “Cross-section of a tumor”, it is better to show the pathological changes (changes observed by HE staining ) in these groups such as Tumor, R1-t, R1-h and HT group.
3) Since genes changes including M2 were examined to be expressed differently in these groups, it needs to be confirmed by IHC method or other methods in these groups.
4) In Table 2, some selected UP and DOWN genes should be better listed.
5) The description in some parts of this article is confusing. For example, the “S” in Figure 2; The “T S” in Figure 3; Line 234, others shown in “Figures 5”? it might be Figure 4? The authors need to carefully check all the parts in this paper.
6) It is interesting that such as “ECM remodeling”, “The direct interaction of cancer cells with the immune cells” were mentioned in this study. Indeed, cancer cells and TME is considered as a pathological ecosystem, and pathological changes during the development of human diseases including cancer should be thought as “Ecological pathology” (Theranostics 2023; 13(5):1607-1631. https://www.thno.org/v13p1607.htm). Based on this concept, the “pseudocapsule” should reflect the long-term ecological process of the crosstalk between tumor and stromal cells. Such advanced view might be helpful for this paper.
Author Response
Reviewer 3:
An interesting study to show that genomic and transcriptomic pseudocapsules of prospective sarcomas were analyzed and revealed to be correlated with a higher risk of recurrence after surgery. Some point should be noted as below.
1) The title should be better as “Analysis of the peritumoral tissue unveils cellular changes associated with a high risk of recurrence in soft-tissue sarcoma”.
We would like to thank the reviewer and we changed the title with adding “in soft-tissue
sarcoma."
2) Instead of Figure 1 “Cross-section of a tumor”, it is better to show the pathological changes (changes observed by HE staining ) in these groups such as Tumor, R1-t, R1-h and HT group.
We have replaced the photograph of the tumor specimen by a histological slide as described above.
3) Since genes changes including M2 were examined to be expressed differently in these groups, it needs to be confirmed by IHC method or other methods in these groups.
We have added an IHC complentary analysis with M2 examination in Figures 9.
4) In Table 2, some selected UP and DOWN genes should be better listed.
We have replaced the GO alone by GO associated with significant genes involved.
5) The description in some parts of this article is confusing. For example, the “S” in Figure 2; The “T S” in Figure 3; Line 234, others shown in “Figures 5”? it might be Figure 4? The authors need to carefully check all the parts in this paper.
Figure 2 was modified with HT, Figure 3 also with T, R1 and HT and in the text, we modified the line 234, « shown in Figure 4 ».
6) It is interesting that such as “ECM remodeling”, “The direct interaction of cancer cells with the immune cells” were mentioned in this study. Indeed, cancer cells and TME is considered as a pathological ecosystem, and pathological changes during the development of human diseases including cancer should be thought as “Ecological pathology” (Theranostics 2023; 13(5):1607-1631. https://www.thno.org/v13p1607.htm). Based on this concept, the “pseudocapsule” should reflect the long-term ecological process of the crosstalk between tumor and stromal cells. Such advanced view might be helpful for this paper.
It has been done and referred in the discussion.
Round 2
Reviewer 3 Report
The authors have made good modifications according to the review suggestions.
