Osteopontin Regulates Treg Cell Stability and Function with Implications for Anti-Tumor Immunity and Autoimmunity
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Flow Cytometry
2.3. Treg Cell ISOLATION, In-Vitro Stimulation and Suppression Assay
2.4. Cell Transfer Model of Colitis—In Vivo Suppression Assay
2.5. Histology and Assessment of Intestinal Inflammation
2.6. B16-F10 Melanoma Model
2.7. Quantitative PCR
2.8. Statistics
3. Results
3.1. Opn-Deficient Foxp3+ Tregs Have Impaired In Vivo Function
3.2. Opn-Deficient Foxp3+ Tregs Exhibit an Unstable Phenotype
3.3. Mice with Opn-Deficient Foxp3+ Tregs Have Enhanced Anti-Tumor Immunity and Reduced Tumor Burden Associated with an Unstable Treg Phenotype
3.4. Enhanced Anti-Tumor Immunity in Opnfl/fl Foxp3YFP-Cre Is Treg Intrinsic
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vakrakou, A.G.; Kourepini, E.; Skordos, I.; Nieto, N.; Panoutsakopoulou, V.; Paschalidis, N. Osteopontin Regulates Treg Cell Stability and Function with Implications for Anti-Tumor Immunity and Autoimmunity. Cancers 2024, 16, 2952. https://doi.org/10.3390/cancers16172952
Vakrakou AG, Kourepini E, Skordos I, Nieto N, Panoutsakopoulou V, Paschalidis N. Osteopontin Regulates Treg Cell Stability and Function with Implications for Anti-Tumor Immunity and Autoimmunity. Cancers. 2024; 16(17):2952. https://doi.org/10.3390/cancers16172952
Chicago/Turabian StyleVakrakou, Aigli G., Evangelia Kourepini, Ioannis Skordos, Natalia Nieto, Vily Panoutsakopoulou, and Nikolaos Paschalidis. 2024. "Osteopontin Regulates Treg Cell Stability and Function with Implications for Anti-Tumor Immunity and Autoimmunity" Cancers 16, no. 17: 2952. https://doi.org/10.3390/cancers16172952