Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Synthesis of Compounds under Study
2.2. Reagents for Biological Evaluations
2.3. Culture Cell Lines
2.4. Viability Assay
2.5. Cell Migration Assay
2.6. Western Blot Assay
2.7. Determination of mRNA Expression by RT-qPCR
Gene | Forward Primer | Reverse Primer |
VEGFA | 5′-CCAGGGTCTCGATTGGATGG-3′ | 5′-GCAGAATCATCACGAAGTGGT-3′ |
MMP-2 | 5′-TCCTGGCAATCCCTTTGTATGTT-3′ | 5′-GTTTCCGCTGCATCCAGACTT-3′ |
MMP-9 | 5′-ACCCGAGTTGGAACCACGAC-3′ | 5′-CATTCAGGGAGACGCCCATT-3′ |
RPLP0 | 5′-CGTCCTCGTGGAAGTGACAT-3′ | 5′-CATGGTGTTCTTGCCCATCAG-3′ |
2.8. Analysis of Drug Combinations through Isobolograms
2.9. Annexin V/Propidium Iodide Staining
2.10. Statistical Analysis
3. Results
3.1. New Synthetic Route of Lipophilic Cation Derived from Gallic and Gentisic Acid
3.2. The Lipophilic Cations Derived from Gallic and Gentisic Acid Trigger a Higher Viability Inhibition than 5-Fluorouracil
3.3. Effects of the Combination of the Lipophilic Cation Derived from Gentisic Acid with 5-Fluorouracil on Metastatic Cells
3.4. Antimigratory Effects of Lipophilic Cations Derived from Gallic and Gentisic Acid in Metastatic Cells
3.5. Effects of Lipophilic Cation Derived from Gallic and Gentisic Acid on the Proliferation and Migration Signaling Pathway in Metastatic Cells
3.6. Effects of the Lipophilic Cations Derived from Gallic and Gentisic Acid on the Metabolic Signaling Pathway in Metastatic Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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IC50 (µM) | ||||||
---|---|---|---|---|---|---|
SW620 Cell Line | CT26 Cell Line | |||||
Compounds | 24 h | 48 h | 72 h | 24 h | 48 h | 72 h |
TPP+C10 | 2.0 ± 0.1 | 2.2 ± 0.5 | 4,0 ± 0,6 | 15.9 ± 0.6 | 10.4 ± 0.9 | 9.1 ± 0.7 |
GA-TPP+C10 | 1.6 ± 0.1 | 1,4 ± 0,3 | 3.0 ± 0.3 | 10.7 ± 1.0 | 9.1 ± 0.3 | 7.5 ± 0.1 |
IC50 (µM) | ||||||
---|---|---|---|---|---|---|
SW620 Cell Line | COLO205 Cell Line | |||||
Compound | 24 h | 48 h | 72 h | 24 h | 48 h | 72 h |
5FU | >200 | >200 | 125.1 ± 29.1 | 68.1 ± 4.8 | 34.8 ± 5.4 | 7.8 ± 2.1 |
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Suárez-Rozas, C.; Jara, J.A.; Cortés, G.; Rojas, D.; Araya-Valdés, G.; Molina-Berrios, A.; González-Herrera, F.; Fuentes-Retamal, S.; Aránguiz-Urroz, P.; Campodónico, P.R.; et al. Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route. Cancers 2024, 16, 2980. https://doi.org/10.3390/cancers16172980
Suárez-Rozas C, Jara JA, Cortés G, Rojas D, Araya-Valdés G, Molina-Berrios A, González-Herrera F, Fuentes-Retamal S, Aránguiz-Urroz P, Campodónico PR, et al. Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route. Cancers. 2024; 16(17):2980. https://doi.org/10.3390/cancers16172980
Chicago/Turabian StyleSuárez-Rozas, Cristian, José Antonio Jara, Gonzalo Cortés, Diego Rojas, Gabriel Araya-Valdés, Alfredo Molina-Berrios, Fabiola González-Herrera, Sebastián Fuentes-Retamal, Pablo Aránguiz-Urroz, Paola Rossana Campodónico, and et al. 2024. "Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route" Cancers 16, no. 17: 2980. https://doi.org/10.3390/cancers16172980