Significance of P53-Binding Protein 1 as a Novel Molecular Histological Marker for Hypopharyngeal Squamous Neoplasms
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Histological Evaluation
2.3. Immunofluorescence Analysis
2.4. Quantification of 53BP1 Nuclear Expression Pattern
2.5. Statistical Analysis
3. Results
3.1. Patient Characteristics
3.2. Progressive Increments in 53BP1 Nuclear Foci at the Tumor Surface during Hypopharyngeal Carcinogenesis
3.3. Association of 53BP1 Nuclear Foci with Tumor Stage and Depth
3.4. The Presence of 53BP1 Nuclear Foci at the Invasive Front Is Inversely Correlated with Lymphovascular Invasion in HPSCC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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n = 39 | |
---|---|
Sex | |
Male, n (%) | 36 (92%) |
Female, n (%) | 3 (8%) |
Age, mean ± SD | 66 ± 7.62 |
Multifocal, n (%) | 6 (15%) |
Metachronous, n (%) | 10 (26%) |
History of pharyngeal/esophagus cancer, n (%) | 26 (67%) |
MCV, mean ± SD | 91.8 ± 12.0 |
γGTP, median [95%CI] | 27 [19–41] |
Alcohol, g/day, median [95%CI] | 50 [62–70] |
Alcohol, g/year, mean ± SD | 42 ± 14.7 |
Smoking pack years, mean ± SD | 44 ± 32.1 |
cTNM, n (%) | |
---|---|
cTis/1/2/3/4 | 6 (15)/19 (48)/7 (17)/3 (7)/4 (10) |
cN 0/1/2b/2c/3b | 32 (82)/3 (7)/1 (2)/2 (5)/1 (2) |
cM 0/1 | 38 (97)/1 (2) |
cStage 0/I/II/III/IVA/IVB/IVC | 6 (15)/17 (43)/4 (10)/5 (12)/5 (12)/1 (2)/1 (2) |
Lympho-vascular invasion | |
Ly+, n (%) | 9 (23) |
v+, n (%) | 8 (20) |
Ly and v, +, n (%) | 5 (13) |
Follow up rate, n (%) | 22 (56) |
Logistic Analysis | |||||||
---|---|---|---|---|---|---|---|
Factors | OR | 95%CI | p | Sensitivity | Specificity | Cutoff | AUC |
Unstable expression | 1.495 | 1.26–1.78 | <0.0001 | 0.789 | 0.865 | 3.251 | 0.858 |
n ≥ 3 | 2.13 | 1.49–3.05 | <0.0001 | 0.816 | 0.769 | 0.787 | 0.837 |
LF | 1.816 | 1.37–2.40 | <0.0001 | 0.868 | 0.769 | 1.46 | 0.834 |
Ki67 | 1.084 | 0.99–1.19 | 0.0845 | 0.447 | 0.827 | 10.784 | 0.629 |
53BP1/Ki67 | 1.864 | 1.25–2.79 | 0.0025 | 0.605 | 0.808 | 0.96 | 0.747 |
OR | 95%CI | p-Value | ||
---|---|---|---|---|
Unstable 53BP1 (n ≥ 3 + LF) at surface | 1.56107 | 1.276307 | 1.909368 | <0.0001 |
Sex (male/female) | 0.69938 | 0.093741 | 5.217704 | 0.7273 |
Age | 1.002435 | 0.922729 | 1.089025 | 0.9541 |
Multifocal | 1.430849 | 0.273848 | 7.476156 | 0.6711 |
Metachronous | 2.117932 | 0.525765 | 8.531632 | 0.2911 |
History of pharyngeal/esophagus cancer | 0.371583 | 0.098147 | 1.406806 | 0.1450 |
Alcohol, g/day | 0.996389 | 0.988839 | 1.003997 | 0.3513 |
Smoking pack years | 1.001769 | 0.984427 | 1.019417 | 0.8428 |
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Kawasaki-Inomata, H.; Tabuchi, M.; Norimatsu, K.; Honda, T.; Matsuda, K.; Hashiguchi, K.; Yamaguchi, N.; Nishi, H.; Kumai, Y.; Nakashima, M.; et al. Significance of P53-Binding Protein 1 as a Novel Molecular Histological Marker for Hypopharyngeal Squamous Neoplasms. Cancers 2024, 16, 2987. https://doi.org/10.3390/cancers16172987
Kawasaki-Inomata H, Tabuchi M, Norimatsu K, Honda T, Matsuda K, Hashiguchi K, Yamaguchi N, Nishi H, Kumai Y, Nakashima M, et al. Significance of P53-Binding Protein 1 as a Novel Molecular Histological Marker for Hypopharyngeal Squamous Neoplasms. Cancers. 2024; 16(17):2987. https://doi.org/10.3390/cancers16172987
Chicago/Turabian StyleKawasaki-Inomata, Hiroko, Maiko Tabuchi, Kiyuu Norimatsu, Tetsuro Honda, Katsuya Matsuda, Keiichi Hashiguchi, Naoyuki Yamaguchi, Hideaki Nishi, Yoshihiko Kumai, Masahiro Nakashima, and et al. 2024. "Significance of P53-Binding Protein 1 as a Novel Molecular Histological Marker for Hypopharyngeal Squamous Neoplasms" Cancers 16, no. 17: 2987. https://doi.org/10.3390/cancers16172987