Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients’ Cohort and Sample Collection
2.2. RNA Extraction and miRNA Expression Analysis
2.3. Statistical Analysis
3. Results
3.1. Clinical–Pathologic Characteristics of the Patients‘Cohort
3.2. miRNA Expression Analysis
3.3. Associations between miRNA Expression and Clinical–Pathologic Characteristics of OSCC Patients
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patient | Gender | Age | Smoker | Alcohol Consumer | Personal History of Cancer | Type of Lesions | Stage * | Localization | Treatment |
---|---|---|---|---|---|---|---|---|---|
Group 1 | |||||||||
1 | M | 64 | yes | no | no | OSCC (G2) | 1 | AR | surgical resection |
2 | F | 80 | yes | no | yes | OSCC (G2) | 4 | BM | surgical resection + radiotherapy |
3 | M | 73 | no | no | no | OSCC (G2) | 3 | tongue | surgical resection + radiotherapy |
4 | M | 55 | no | no | no | OSCC (G2) | 4 | tongue | surgical resection + radiotherapy |
5 | F | 61 | yes | no | no | OSCC (G2) | 4 | tongue | surgical resection + radiotherapy |
6 | M | 68 | yes | yes | no | OSCC (G2) | 3 | FOM | surgical resection + radiotherapy |
7 | F | 78 | no | no | yes | OSCC (G2) | 1 | tongue | surgical resection |
8 | M | 52 | yes | yes | no | OSCC (G2) | 4 | tongue | surgical resection + radiotherapy |
9 | F | 81 | no | no | no | OSCC (G2) | 3 | tongue | surgical resection + radiotherapy |
10 | M | 68 | no | no | yes | OSCC (G2) | 1 | BM | surgical resection |
11 | F | 65 | no | no | no | OSCC (G2) | 1 | tongue | surgical resection |
12 | M | 75 | yes | no | no | OSCC (G2) | 4 | AR | surgical resection + radiotherapy |
13 | F | 79 | no | no | no | OSCC (G2) | 4 | tongue | surgical resection + radiotherapy |
14 | M | 84 | no | no | no | OSCC (G2) | 2 | AR | surgical resection |
Group 2 | |||||||||
1 | M | 77 | yes | no | yes | PVL with mild dysplasia | / | tongue, gingiva, hard palate, BM | laser ablation and follow-up |
2 | F | 70 | no | no | no | OLP with mild dysplasia | / | BM | topical corticosteroids |
3 | M | 65 | no | no | no | PVL with moderate dysplasia | / | hard palate and tongue | laser ablation and follow-up |
4 | F | 58 | yes | no | no | OLL with mild dysplasia | / | BM | surgical resection and removal of dental material |
5 | M | 79 | no | no | yes | OLP with mild dysplasia | / | BM and tongue | systemic corticosteroids |
6 | F | 57 | no | no | no | OLP with mild dysplasia | BM | topical corticosteroids | |
Group 3 | |||||||||
1 | M | 61 | yes | no | no | Healthy control | / | / | |
2 | F | 72 | no | no | no | Healthy control | / | / | |
3 | F | 48 | yes | no | no | Healthy control | / | / | |
4 | M | 52 | no | no | no | Healthy control | / | / | |
5 | F | 80 | no | no | no | Healthy control | / | / |
Variable | Analysis of Variance; Statistical Significance: p ≤ 0.05 | |||||||
---|---|---|---|---|---|---|---|---|
SS Effect | df Effect | MS Effect | SS Error | df Error | MS Error | F | p | |
miR-21_s | 0.491583 | 2 | 0.245791 | 13.70107 | 22 | 0.622776 | 0.394671 | 0.678578 |
miR-31_s | 0.043059 | 2 | 0.02153 | 4.02928 | 22 | 0.183149 | 0.117552 | 0.889649 |
miR-138_s * | 0.538658 | 2 | 0.269329 | 1.0629 | 22 | 0.048313 | 5.574616 | 0.011001 |
miR-145_s | 1.553145 | 2 | 0.776573 | 18.83423 | 22 | 0.856101 | 0.907104 | 0.418265 |
miR-184_s | 0.035187 | 2 | 0.017594 | 0.15721 | 22 | 0.007146 | 2.4621 | 0.108412 |
miR-424_s * | 0.046202 | 2 | 0.023101 | 0.14609 | 22 | 0.00664 | 3.478801 | 0.04867 |
Variable | Analysis of Variance; Statistical Significance: p ≤ 0.05 | |||||||
---|---|---|---|---|---|---|---|---|
SS Effect | df Effect | MS Effect | SS Error | df Error | MS Error | F | p | |
miR-21_b | 0.248505 | 2 | 0.124252 | 7.12042 | 22 | 0.323656 | 0.383903 | 0.685672 |
miR-31_b | 0.000243 | 2 | 0.000121 | 0.00799 | 22 | 0.000363 | 0.33446 | 0.719301 |
miR-138_b | 0.006047 | 2 | 0.003023 | 0.2178 | 22 | 0.0099 | 0.305389 | 0.73991 |
miR-145_b | 0.995649 | 2 | 0.497824 | 28.46033 | 22 | 1.293651 | 0.384821 | 0.685064 |
miR-184_b | 0.000011 | 2 | 0.000006 | 0.0007 | 22 | 0.000032 | 0.17839 | 0.837814 |
miR-424_b | 1.12825 | 2 | 0.564125 | 32.49662 | 22 | 1.477119 | 0.381909 | 0.686995 |
Variable | Analysis of Variance; Statistical Significance: p ≤ 0.05 | |||||||
---|---|---|---|---|---|---|---|---|
SS Effect | df Effect | MS Effect | SS Error | df Error | MS Error | F | p | |
miR-21_s | 0.775676 | 1 | 0.775676 | 3.04138 | 12 | 0.253448 | 3.060494 | 0.105722 |
miR3-1_s | 0.198984 | 1 | 0.198984 | 0.86327 | 12 | 0.07194 | 2.765992 | 0.122164 |
miR-138_s | 0.000929 | 1 | 0.000929 | 0.09657 | 12 | 0.008048 | 0.11545 | 0.739896 |
miR-145_s | 0.579964 | 1 | 0.579964 | 13.23836 | 12 | 1.103197 | 0.525712 | 0.482315 |
miR-184_s | 0.001046 | 1 | 0.001046 | 0.01674 | 12 | 0.001395 | 0.749777 | 0.403527 |
miR-424_s * | 0.006783 | 1 | 0.006783 | 0.01576 | 12 | 0.001314 | 5.163991 | 0.042251 |
miR-21_b | 0.886818 | 1 | 0.886818 | 6.22825 | 12 | 0.519021 | 1.708637 | 0.215655 |
miR-31_b | 0.000552 | 1 | 0.000552 | 0.00663 | 12 | 0.000552 | 0.999868 | 0.33708 |
miR-138_b | 0.026883 | 1 | 0.026883 | 0.1904 | 12 | 0.015867 | 1.694303 | 0.217469 |
miR-145_b | 3.674721 | 1 | 3.674721 | 24.78074 | 12 | 2.065061 | 1.779473 | 0.206979 |
miR-184_b | 0.00008 | 1 | 0.00008 | 0.00061 | 12 | 0.000051 | 1.579332 | 0.232769 |
miR-424_b | 4.285125 | 1 | 4.285125 | 28.205 | 12 | 2.350416 | 1.823134 | 0.201857 |
Variable | Analysis of Variance; Statistical Significance: p ≤ 0.05 | |||||||
---|---|---|---|---|---|---|---|---|
SS Effect | df Effect | MS Effect | SS Error | df Error | MS Error | F | p | |
miR-21_s | 0.002989 | 1 | 0.002989 | 0.619293 | 12 | 0.051608 | 0.057913 | 0.813888 |
miR-31_s | 0.00564 | 1 | 0.00564 | 0.266388 | 12 | 0.022199 | 0.254057 | 0.623359 |
miR-138_s | 0.000683 | 1 | 0.000683 | 0.039585 | 12 | 0.003299 | 0.206913 | 0.657317 |
miR-145_s * | 0.312089 | 1 | 0.312089 | 0.529068 | 12 | 0.044089 | 7.078613 | 0.02077 |
miR-184_s | 0.000106 | 1 | 0.000106 | 0.00786 | 12 | 0.000655 | 0.161932 | 0.69446 |
miR-424_s | 0.001124 | 1 | 0.001124 | 0.008707 | 12 | 0.000726 | 1.548489 | 0.237115 |
miR-21_b | 0.014053 | 1 | 0.014053 | 0.632323 | 12 | 0.052694 | 0.266694 | 0.614942 |
miR-31_b | 0.000092 | 1 | 0.000092 | 0.003164 | 12 | 0.000264 | 0.348916 | 0.565687 |
miR-138_b | 0.001348 | 1 | 0.001348 | 0.073555 | 12 | 0.00613 | 0.219903 | 0.647519 |
miR-145_b | 0.020575 | 1 | 0.020575 | 0.83404 | 12 | 0.069503 | 0.296022 | 0.596352 |
miR-184_b | 0.000006 | 1 | 0.000006 | 0.000318 | 12 | 0.000027 | 0.229319 | 0.640637 |
miR-424_b | 0.016404 | 1 | 0.016404 | 0.852331 | 12 | 0.071028 | 0.230951 | 0.639462 |
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Rocchetti, F.; Tenore, G.; Macali, F.; Vicidomini, T.; Podda, G.M.; Fantozzi, P.J.; Silvestri, V.; Porzio, V.; Valentini, V.; Ottini, L.; et al. Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders. Cancers 2024, 16, 2990. https://doi.org/10.3390/cancers16172990
Rocchetti F, Tenore G, Macali F, Vicidomini T, Podda GM, Fantozzi PJ, Silvestri V, Porzio V, Valentini V, Ottini L, et al. Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders. Cancers. 2024; 16(17):2990. https://doi.org/10.3390/cancers16172990
Chicago/Turabian StyleRocchetti, Federica, Gianluca Tenore, Federica Macali, Teresa Vicidomini, Gian Marco Podda, Paolo Junior Fantozzi, Valentina Silvestri, Virginia Porzio, Virginia Valentini, Laura Ottini, and et al. 2024. "Expression Analysis of Circulating microRNAs in Saliva and Plasma for the Identification of Clinically Relevant Biomarkers for Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders" Cancers 16, no. 17: 2990. https://doi.org/10.3390/cancers16172990