Application of Bio-Inspired Gold Nanoparticles as Advanced Nanomaterial in Halt Nociceptive Pathway and Hepatotoxicity via Triggering Antioxidation System
, Amal M. Al Mohaimeed 4, Qirat Naz 5 and Asif Kamal 6,*
Round 1
Reviewer 1 Report
Authors have done extensive analysis however this MS is publishable only after some major revisions. Here are some revisions that should be added in the MS.
1. It would be great if authors could provide TEM analysis of samples (if available). This will give a better picture of morphology of gold nanoparticles.
2. The GCMS chromatogram is full of background noise, I think the authors should take a better chromatogram.
3. Mass Spectra of all the identified compounds should be provided in supporting information.
4. Authors have mentioned green method as such some latest articles must be cited.
Examples:
https://doi.org/10.1016/j.mtchem.2022.101016
https://doi.org/10.1038/s41598-022-11811-7
https://doi.org/10.1016/j.jscs.2022.101419
https://doi.org/10.1016/j.msec.2020.111515
Author Response
Answers to Reviewers’ comments
(Round 1) Reviewer 1
- It would be great if authors could provide TEM analysis of samples (if available). This will give a better picture of morphology of gold nanoparticles.
Response: TEM image been added
2. The GCMS chromatogram is full of background noise, I think the authors should take a better chromatogram.
Response: The chromatogram is system generated and is non-editable. As the GC-MS data is displayed in table along with the mass spectra of each compound so the chromatogram may be deleted if suggested by the reviewer.
- Mass Spectra of all the identified compounds should be provided in supporting information.
Response: Mass Spectra of all identified compounds are added to the table
- Authors have mentioned green method as such some latest articles must be cited.
Examples:
https://doi.org/10.1016/j.mtchem.2022.101016
https://doi.org/10.1038/s41598-022-11811-7
https://doi.org/10.1016/j.jscs.2022.101419
https://doi.org/10.1016/j.msec.2020.111515
Reviewer 2 Report
The submitted manuscript (ID: catalysts-2299216) described the synthesis of gold nanoparticles (GNS) using Euphorbia wallichii (REEW) as raw material. Experiments have proved that GNS has tremendouspotential for curing degenerative/metabolic diseases caused as a result of oxidative stresses.But the language of this manuscript is illogical, and the innovation can’t meet the requirements of this journal. In short, the quality of the article does not meet the requirements of this journal, and it is recommended to transfer to other journals. In addition, a number of issues need to be clarified before the manuscript can be submitted to another journal.
1. The manuscript is not innovative enough and the logic is confused.
2. It is suggested that the summary section be revised to make it easier to read.
3. The references are too old.
4. In lines 51-52, the first appearance of NPs should have its full name, right? Please reconfirm whether the writing is correct!
5. In line 70, ditto, please confirm whether the full name of the GNS here is required.
6. In lines 128-129, please check again to ensure that there are no errors in the format.
7. In lines 181-184, unpopular low temperature oxidation process? Are all low-temperature oxidation processes unpopular? At the same time, please make sure that references are needed here?
8. In lines 184-187, please confirm again that this conclusion is consistent with the results of figure 6?
9. In Lines 187-189, where is the data for FTIR? Please indicate.
10. In lines 189-190, please check again if there is a problem with the expression of this sentence?
11. In lines 194-195, the experiment in this section explores the antioxidant activity of REEW-GNS, right? So, how do you prove that REEW-GNS clears ROSs?
12. Figure 7 shows that ascorbic acid is better than REEW GNSS, right? Please explain and reconfirm the accuracy of the conclusions of this section.
13. Please confirm that figure 9 is consistent with the conclusions described in this section? Does the ordinate of the picture indicate the antidiabetic activity or the blood glucose level?
14. In figure 10.Where is the 300mg / kg GNS data? I didn't read it in the figure.
Author Response
Answers to Reviewers’ comments
(Round 1) Reviewer 2
Response: The mentioned articles were informative and are cited in MS as per suggestion.
- The manuscript is not innovative enough and the logic is confused.
Response: As the synthesis of nano-drugs using phytochemical is a new frontier in modern therapeutics. As the current manuscript optimized the conditions for the synthesis of gold based nano-drug of phytochemicals from euphorbia wallichii and its possible invitro and invivo application as heptoprotactive, analgesic and antispasmodic agent which has not been reported earlier so, the manuscript has enough novelty in terms of its findings.
- It is suggested that the summary section be revised to make it easier to read.
Response: Summery section been revised to a more simple language.
- The references are too old.
Response: old references been replaced with recent available literature in the subject matter.
- In lines 51-52, the first appearance of NPs should have its full name, right? Please reconfirm whether the writing is correct!
Response: The short form NPs; has been replaced with its full form i.e. nanoparticles.
- In line 70, ditto, please confirm whether the full name of the GNS here is required.
Response: The full name of GNs as gold nanoparticles been added
- In lines 128-129, please check again to ensure that there are no errors in the format.
Response: The mentioned lines are reformatted.
- In lines 181-184, unpopular low temperature oxidation process? Are all low-temperature oxidation processes unpopular? At the same time, please make sure that references are needed here?
Response: Thanks you for correcting me in this regard. As not all the low-temperature oxidation processes unpopular and some are of commercial advantage, so the word undesired has been omitted from the sentence.
- In lines 184-187, please confirm again that this conclusion is consistent with the results of figure 6?
Response: Thank you for reporting the mistake. The legends of the treatments were wrongly coded in the previous graph which is now corrected and is inline with the conclusion.
- In Lines 187-189, where is the data for FTIR? Please indicate.
Response: The data for FTIR spectroscopy has been explained earlier in characterization section of the MS. In this sentence only the results been related to functional groups discussed earlier.
- In lines 189-190, please check again if there is a problem with the expression of this sentence?
Response: Sentence has been revised
- In lines 194-195, the experiment in this section explores the antioxidant activity of REEW-GNS, right? So, how do you prove that REEW-GNS clears ROSs?
Response: This section of experiment is about scavenging the OH* radical by GNs which was confirmed by the bleaching effect of OH* on phenol red dye. As the OH* radical is a type of ROS which has been scavenged by GNs as evident from the figure thus exhibited antioxidant potential against OH* radical.
- Figure 7 shows that ascorbic acid is better than REEW GNSS, right? Please explain and reconfirm the accuracy of the conclusions of this section.
Response: A comparative antioxidant effect of ascorbate and REEW-GNs has been added to the result.
- Please confirm that figure 9 is consistent with the conclusions described in this section? Does the ordinate of the picture indicate the antidiabetic activity or the blood glucose level?
Response: Yes the conclusion is in line with figure 9. The ordinate of the picture representing the hypoglycemia in percent (reduction in blood glucose level) produced by various treatments compared to the control animals (hyperglycemic without treatment)
- In figure 10.Where is the 300mg / kg GNS data? I didn't read it in the figure.
Response: Thank you for highlighting the mistake. The legends of the figure are corrected.
Reviewer 3 Report
Title “Application of Bio-Inspired Gold nanoparticles as Advanced nanomaterial in Halt Nociceptive Pathway and Hepatotoxicity via Triggering Antioxidation System”.
The authors synthesized the gold nanoparticles through a simple and green method. And there are some questions should be solved before acceptation.
(1) Please provide the proofs or references to support your conclusions ” for the the absorption of the sample above 600 nm was reasonably lower, indicating the particles in solution did not suffer agglomeration”.
(2) Please add the exact name of the reactants in figure 1 to help the readers to understand the meaning through the figures directly.
(3) Improve the quality of figure 2. And we can not know the crystal structure through SEM analysis. So the fcc structure obtained with SEM is not correct.
(4) Figure 3, why do you have two lines in the standard Au PDF?
(5) Figure 4, normally the peak of functional group will shift a little bit when it binds with some nanoparticles, please provide more detailed information for your IR test.
(6) The conclusions should be rewritten, since it did not reflect the main contents/conclusions of this paper.
(7) In addition, the format and English should be polished, for example, the abbreviation of “gold nanoparticles” should be GNs.
Author Response
Answers to Reviewers’ comments
(Round 1) Reviewer 3
- Please provide the proofs or references to support your conclusions ” for the the absorption of the sample above 600 nm was reasonably lower, indicating the particles in solution did not suffer agglomeration”.
Response: The proof along with reference has been added in support of the conclusion about the Plasmon rule of gold agglomeration.
- Please add the exact name of the reactants in figure 1 to help the readers to understand the meaning through the figures directly.
Response: Exact name of the reagents are added to the legends of the figure 1.
- Improve the quality of figure 2. And we cannot know the crystal structure through SEM analysis. So the fcc structure obtained with SEM is not correct.
Response: The quality of figure 2 has been improved. Yes sir you rightly said the crystal geometry is indicated by XRD analysis and not through electron microscopy and the correction been made accordingly.
- Figure 3, why do you have two lines in the standard Au PDF?
Response: The two closed intensities in the standard XRD spectra of crystallographic search match correspond to the normalized intensity values at 100% and 0% diffraction respectively. These values are used to normalized the intensity values for other peaks in diffraction pattern.
- Figure 4, normally the peak of functional group will shift a little bit when it binds with some nanoparticles, please provide more detailed information for your IR test.
Response: Thank you for highlighting this important point. Yes there is a shift in IR peaks but in the figure we just replicated the peak positions of REEW to GNs. Now we relocated the peak position of GNs independently and find a slight shifts in some of the peaks.
- The conclusions should be rewritten, since it did not reflect the main contents/conclusions of this paper.
Response: Conclusion has been restructured in light of the findings of the MS.
- In addition, the format and English should be polished, for example, the abbreviation of “gold nanoparticles” should be GNs.
Response: The language has been thoroughly reviewed and all the abbreviations of gold nanoparticles” were made as GNs.
Round 2
Reviewer 1 Report
As the authors have addressed all the comments, this MS can now be accepted.
Anshuman
Reviewer 3 Report
can be accepted
