Personalised 3D Printed Fast-Dissolving Tablets for Managing Hypertensive Crisis: In-Vitro/In-Vivo Studies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Filaments
2.3. Physicochemical Characterisation of Filaments
2.3.1. Determination of Drug Loading
2.3.2. Differential Scanning Calorimetry (DSC)
2.3.3. X-ray Powder Diffraction (XRPD)
2.3.4. Scanning Electron Microscopy (SEM)
2.3.5. Mechanical Testing
2.4. Fabrication of 3D Printed Tablets
2.5. Characterisation of 3D Printed Tablets
2.5.1. Geometrical, Porosity, and Morphological Assessment of 3D Printed Tablets
2.5.2. Determination of Tablet Hardness and Friability
2.5.3. 3D parametric Surface Texture Analysis
2.5.4. In-Vitro Disintegration Testing
2.5.5. In-Vitro Captopril Dissolution Investigation
2.5.6. In-Vivo Pharmacokinetic Investigation
3. Results and Discussion
3.1. Development and Characterisation of Filaments
3.2. Development and Characterisation of 3D Printed Matrix Tablets
3.2.1. Content Uniformity, Hardness, Porosity, and Friability of Printed Tablets
3.2.2. Morphological Analysis of Printed Tablets
3.2.3. 3D Parametric Surface Texture Analysis of 3D Printed Tablets
3.2.4. In-Vitro Disintegration and Dissolution Studies
3.2.5. In-Vivo Pharmacokinetic Studies
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Formulation | Tensile Strength (MPa) | Young’s Modulus (MPa) | Stiffness (kN/m) | Drug Loading (%) |
---|---|---|---|---|
F1 | 20.13 ± 0.38 | 1931.5 ± 193.1 | 134.8 ± 11.8 | - |
F2 | 19.81 ± 0.25 | 1727.8 ± 434.3 | 111.7 ± 26.6 | 98.65 ± 0.77 |
F3 | 19.95 ± 0.34 | 1468.9 ± 109.8 | 89.97 ± 6.72 | 97.43 ± 1.2 |
F4 | 20.16 ± 0.26 | 1475.3 ± 279.6 | 86.48 ± 5.2 | 96.50 ± 0.35 |
Formulation | Weight (mg) | Diameter (mm) | Height (mm) | Drug Loading (%) | Breaking Strength (N) | Porosity (%) | Friability (%) |
---|---|---|---|---|---|---|---|
F1 | 175.0 ± 0.75 | 7.00 ± 0.10 | 2.13 ± 0.11 | - | 385.1 ± 11.5 | 1.6 ± 0.6 | 0 |
F2 | 175.2 ± 0.80 | 7.05 ± 0.12 | 2.15 ± 0.15 | 97.15 ± 1.2 | 411.3 ± 19.3 | 2.4 ± 0.5 | 0 |
F3 | 175.1 ± 1.1 | 7.02 ± 0.15 | 2.20 ± 0.10 | 96.77 ± 0.8 | 406.4 ± 21.3 | 2.1 ± 0.3 | 0 |
F4 | 175.4 ± 0.5 | 7.10 ± 0.18 | 2.22 ± 0.12 | 98.10 ± 1.1 | 409.9 ± 6.98 | 1.7 ± 0.4 | 0 |
Parameter | F1 | F2 | F3 | F4 |
---|---|---|---|---|
Sa (μm) | 20.11 ± 4.25 | 24.19 ± 2.14 | 22.16 ± 1.54 | 25.19 ± 3.31 |
Sq (μm) | 25.29 ± 6.84 | 30.15 ± 3.11 | 31.25 ± 2.88 | 33.22 ± 2.44 |
Sz (μm) | 57.83 ± 5.92 | 61.95 ± 6.86 | 67.59 ± 5.12 | 75.95 ± 6.11 |
Sp (μm) | 24.16 ± 4.21 | 39.26 ± 2.49 | 41.26 ± 4.16 | 44.29 ± 6.12 |
Sv (μm) | 33.67 ± 5.22 | 22.69 ± 7.15 | 26.33 ± 7.19 | 31.66 ± 6.05 |
Sku (μm) | 4.21 ± 0.95 | 4.55 ± 1.11 | 4.88 ± 0.89 | 5.11 ± 1.21 |
Ssk (μm) | −0.414 ± −0.48 | −0.29 ± −0.11 | −0.42 ± −0.15 | −0.36 ± −0.10 |
Parameter | Standard | F2 | F3 | F4 |
---|---|---|---|---|
Tmax (h) | 2.00 ± 0.00 | 3.67 ± 0.52 | 2.00 ± 0.00 | 2.17 ± 0.41 |
Cmax (ng/mL) | 1158.45 ± 71.67 | 1054.12 ± 64.36 | 1117.02 ± 32.46 | 1175.11 ± 21.65 |
AUC0–24h (ng/mL·h) | 6004.88 ± 125.49 | 7039.39 ± 931.58 | 5836.30 ± 102.51 | 4006.75 ± 58.66 |
MRT (h) | 6.51 ± 0.76 | 11.98 ± 1.98 | 7.66 ± 0.28 | 4.70 ± 0.08 |
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Hussain, A.; Mahmood, F.; Arshad, M.S.; Abbas, N.; Qamar, N.; Mudassir, J.; Farhaj, S.; Nirwan, J.S.; Ghori, M.U. Personalised 3D Printed Fast-Dissolving Tablets for Managing Hypertensive Crisis: In-Vitro/In-Vivo Studies. Polymers 2020, 12, 3057. https://doi.org/10.3390/polym12123057
Hussain A, Mahmood F, Arshad MS, Abbas N, Qamar N, Mudassir J, Farhaj S, Nirwan JS, Ghori MU. Personalised 3D Printed Fast-Dissolving Tablets for Managing Hypertensive Crisis: In-Vitro/In-Vivo Studies. Polymers. 2020; 12(12):3057. https://doi.org/10.3390/polym12123057
Chicago/Turabian StyleHussain, Amjad, Faisal Mahmood, Muhammad Sohail Arshad, Nasir Abbas, Nadia Qamar, Jahanzeb Mudassir, Samia Farhaj, Jorabar Singh Nirwan, and Muhammad Usman Ghori. 2020. "Personalised 3D Printed Fast-Dissolving Tablets for Managing Hypertensive Crisis: In-Vitro/In-Vivo Studies" Polymers 12, no. 12: 3057. https://doi.org/10.3390/polym12123057