Novel Approach for Cardioprotection: In Situ Targeting of Metformin via Conductive Hydrogel System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of CSMA and Pam-GelMA
2.3. Cytotoxicity Assay
2.4. Rheological Testing of Hydrogels
2.5. Live/Dead Staining
2.6. Detection of Metf Release
2.7. JC-1 Staining
2.8. ROS Detection
2.9. Establishment of Rat Model
2.10. Echocardiographic Analysis
2.11. Histological Analysis
2.12. Statistical Analysis
3. Results and Discussion
3.1. Preparation of CSMA/Pam-GelMA Hydrogels
3.2. Characterization of CSMA/Pam-GelMA Hydrogels
3.3. Effect of Metf/Hydrogel on ROS-Induced CM Activity
3.4. Effect of Metf/Hydrogel on Heart Structure and Function in Rats with MI
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Tan, Y.; Li, J.; Nie, Y.; Zheng, Z. Novel Approach for Cardioprotection: In Situ Targeting of Metformin via Conductive Hydrogel System. Polymers 2024, 16, 2226. https://doi.org/10.3390/polym16152226
Tan Y, Li J, Nie Y, Zheng Z. Novel Approach for Cardioprotection: In Situ Targeting of Metformin via Conductive Hydrogel System. Polymers. 2024; 16(15):2226. https://doi.org/10.3390/polym16152226
Chicago/Turabian StyleTan, Ying, Jierong Li, Yali Nie, and Zhi Zheng. 2024. "Novel Approach for Cardioprotection: In Situ Targeting of Metformin via Conductive Hydrogel System" Polymers 16, no. 15: 2226. https://doi.org/10.3390/polym16152226