Congruity of the Polymorphisms in the Expressed and Noncoding Parts of the Gli-B1 Locus in Common Wheat
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Alleles at the Gli-B1 Locus and PCR Sequence Length Polymorphism in the Set of Cultivars Studied
3. Results
3.1. Allelic Variation at the Gli-B1 Locus and PCR Product Length Polymorphism among the Cultivars Studied
3.1.1. Allelic Variation
3.1.2. PCR Product Length Polymorphism
3.2. The Correspondence between PCR Product Polymorphism and Allelic Variation at the Gli-B1 Locus
3.3. The Probable Recombinational Nature of the Allele Gli-B1j
3.4. Variation in PCR Product Length among Cultivars Having Identical Alleles at the Gli-B1 Locus
3.5. Polymorphism of the PCR Products in “Zone B” of the Electrophoregram
3.6. The PCR Sequence Corresponds to Genotype, Not to the Label of the Grain Sample under Study
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Cultivar or Grain Sample 1 | Habit | Country | Year | Allele Gli-B1 | Primer | Length 2 | |
---|---|---|---|---|---|---|---|
1 | Bezostaya-1 | w | Russia | 1959 | b | GliB1.1 | 369 |
2 | Gabo | s | Australia | 1942 | b | GliB1.1 | 369 |
3 | Marquis | s | Canada | 1907 | b | GliB1.1 | 369 |
4 | Mironovskaya-808 | w | Ukraine | 1963 | b | GliB1.1 | 369 |
5 | “Kavkaz” 3 | b | GliB1.1 | 369 | |||
6 | Federation | s | Australia | 1901 | i | GliB1.1 | 400 |
7 | Insignia | s | Australia | 1946 | i | GliB1.1 | 400 |
8 | Mentana | s | Italy | 1913 | k | GliB1.1 | 400 |
9 | Pane-247 | w | Spain | 1960 | k | GliB1.1 | 400 |
10 | Titien | w | France | 1985 | m | GliB1.1 | 400 |
11 | Aragon-03 | s, w | Spain | 1940 | o | GliB1.1 | 400 |
12 | Potam-70 | s | Mexico | 1970 | p | GliB1.1 | 400 |
13 | Gazul | s | Spain | 1992 | r | GliB1.1 | 400 |
14 | “Argelato” 3 | m | GliB1.1 | 400 | |||
15 | Cluj-650 | w | Rumania | 1954 | j | GliB1.1 | 400 |
16 | Intensivnaya | i | Kyrgyzstan | 1978 | n | GliB1.1 | 372 |
17 | Goelent | w | France | 1985 | q | GliB1.1 | 372 |
18 | Salmone | w | Italy | 1980 | s | GliB1.1 | 372 |
19 | Cartaya | s | Spain | l | GliB1.1 | - | |
20 | Chinese-Spring | s | China | - | a | GliB1.2 | 415 |
21 | Cajeme-71 | s | Mexico, USA | 1971 | d | GliB1.2 | 409 |
22 | Katepwa | s | Canada | 1981 | d | GliB1.2 | 409 |
23 | Laura | s | Canada | 1986 | d | GliB1.2 | 409 |
24 | Pavon-76 | s | Mexico | 1976 | d | GliB1.2 | 409 |
25 | Rinconada | s | Spain | 1981 | d | GliB1.2 | 409 |
26 | Roblin | s | Canada | 1986 | d | GliB1.2 | 409 |
27 | Suneca | s | Australia | 1981 | d | GliB1.2 | 409 |
28 | Yecora-S | s | Mexico | 1972 | d | GliB1.2 | 409 |
29 | “Inia-66” 3 | d | GliB1.2 | 409 | |||
30 | Diego | s | Spain | 1983 | c | GliB1.2 | 397 |
31 | Prinqual | s | France | 1978 | c | GliB1.2 | 397 |
32 | Siete-Cerros-66 | s | Mexico | 1966 | c | GliB1.2 | 397 |
33 | Escualo | w | Spain | 1981 | e | GliB1.2 | 397 |
34 | Glenlea | s | Canada | 1972 | e | GliB1.2 | 397 |
35 | Arminda | w | Netherlands | 1976 | f | GliB1.2 | 397 |
36 | Cappelle-Desprez | w | France | 1946 | f | GliB1.2 | 397 |
37 | Darius | w | France | 1974 | f | GliB1.2 | 397 |
38 | Recital | w | France | 1986 | f | GliB1.2 | 397 |
39 | Sideral | w | France | 1990 | f | GliB1.2 | 397 |
40 | “Libero” 3 | f | GliB1.2 | 397 | |||
41 | Argelato | s, w | Italy | 1964 | g | GliB1.2 | 397 |
42 | Galahad | w | UK | 1983 | g | GliB1.2 | 397 |
43 | Ardec | s | Belgium | 1979 | h | GliB1.2 | 402 |
44 | Caia | s | Portugal | - | h | GliB1.2 | 403 |
45 | Krasnodonka | w | Ukraine | 1969 | h | GliB1.2 | 401 |
46 | Newcaster | w | USA | 1946 | h | GliB1.2 | 403? 4 |
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Popovych, Y.; Chebotar, S.; Melnik, V.; Rodriguez-Quijano, M.; Pascual, L.; Rogers, W.J.; Metakovsky, E. Congruity of the Polymorphisms in the Expressed and Noncoding Parts of the Gli-B1 Locus in Common Wheat. Agronomy 2020, 10, 1510. https://doi.org/10.3390/agronomy10101510
Popovych Y, Chebotar S, Melnik V, Rodriguez-Quijano M, Pascual L, Rogers WJ, Metakovsky E. Congruity of the Polymorphisms in the Expressed and Noncoding Parts of the Gli-B1 Locus in Common Wheat. Agronomy. 2020; 10(10):1510. https://doi.org/10.3390/agronomy10101510
Chicago/Turabian StylePopovych, Yulia, Sabina Chebotar, Viktor Melnik, Marta Rodriguez-Quijano, Laura Pascual, William John Rogers, and Eugene Metakovsky. 2020. "Congruity of the Polymorphisms in the Expressed and Noncoding Parts of the Gli-B1 Locus in Common Wheat" Agronomy 10, no. 10: 1510. https://doi.org/10.3390/agronomy10101510