The FBA Motif-Containing Protein NpFBA1 Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Culture of Pathogens and Production of Spore Suspension
2.3. RNA Extraction and cDNA Synthesis
2.4. NpFBA1 Cloning and Sequence Analysis
2.5. Subcellular Localization of NpFBA1 Proteins
2.6. Hormone Treatment and Pathogen Infection
2.7. NpFBA1 Gene Expression Vector Constructs and the Acquisition of Transgenic Tobacco
2.8. DNA Extraction from Transgenic and Wild Tobacco
2.9. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
2.10. Leaf Anatomy Observation
2.11. Calculation of Leaf Damage Area after P. parasitica Infection
3. Results
3.1. Identification of the NpFBA1 Gene from Nicotiana plumbaginifolia
3.2. Subcellular Localization of NpFBA1
3.3. NpFBA1 Responds to Pathogen Infection and Exogenous Hormones
3.4. Functional Identification of the NpFBA1 Gene
3.4.1. Effects of the NpFBA1 Gene on Tobacco Growth and Development
3.4.2. Observation of the Leaf Anatomical Structure of WT and Overexpressed Plants
3.4.3. Pathogen Infection Status
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Materials | Leaf Length (cm) | Leaf Width (cm) | Leaf Shape Index (Length/Width) |
---|---|---|---|
WT | 19.33 ± 2.35 | 6.90 ± 0.63 | 2.81 ± 0.03 |
NpFBA1#2 | 16.00 ± 1.75 * | 8.10 ± 0.58 | 2.02 ± 0.01 * |
NpFBA1#9 | 17.80 ± 0.28 | 7.90 ± 0.13 | 2.29 ± 0.02 * |
NpFBA1#17 | 17.16 ± 0.58 | 8.00 ± 0.25 | 2.15 ± 0.01 * |
Materials | Leaf Thickness (µm) | Spongy Tissue Thickness (µm) | Upper Epidermis Thickness (µm) | CTR% | Number of Cells per 1 mm Palisade Tissue |
---|---|---|---|---|---|
WT | 177.25 ± 5.27 | 89.09 ± 1.67 | 10.30 ± 1.78 | 33.87 ± 3.03 | 44.33 ± 5.13 |
NpFBA1#2 | 210.17 ± 18.25 * | 142.66 ± 10.64 ** | 25.47 ± 2.79 ** | 24.10 ± 4.81 * | 57.00 ± 1.73 ** |
NpFBA1#9 | 210.83 ± 10.57 ** | 97.55 ± 3.21 * | 24.14 ± 1.80 ** | 22.72 ± 4.99 * | 54.67 ± 2.08 * |
NpFBA1#17 | 193.16 ± 4.05 * | 128.96 ± 8.61 ** | 22.64 ± 2.95 ** | 19.60 ± 0.67 ** | 70.33 ± 4.16 ** |
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Xie, Z.; Wen, G.; Yang, Y.; Wang, H.; Wang, J.; Lei, C.; Guo, Q.; Dang, J.; Liang, G. The FBA Motif-Containing Protein NpFBA1 Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco. Agronomy 2021, 11, 2478. https://doi.org/10.3390/agronomy11122478
Xie Z, Wen G, Yang Y, Wang H, Wang J, Lei C, Guo Q, Dang J, Liang G. The FBA Motif-Containing Protein NpFBA1 Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco. Agronomy. 2021; 11(12):2478. https://doi.org/10.3390/agronomy11122478
Chicago/Turabian StyleXie, Zhongyi, Guo Wen, Yao Yang, Haiyan Wang, Jinying Wang, Chenggong Lei, Qigao Guo, Jiangbo Dang, and Guolu Liang. 2021. "The FBA Motif-Containing Protein NpFBA1 Causes Leaf Curling and Reduces Resistance to Black Shank Disease in Tobacco" Agronomy 11, no. 12: 2478. https://doi.org/10.3390/agronomy11122478