Genotype by Environment Interaction Effect on Grain Iron and Zinc Concentration of Indian and Mediterranean Lentil Genotypes
, Gyan Prakash Mishra 1,*, Muraleedhar S. Aski 1, Ruchi Bansal 3, Kuldeep Tripathi 3, Arpan Bhowmik 4 and Shiv Kumar 5Round 1
Reviewer 1 Report
Review attached.
Comments for author File: 
Comments.pdf
Author Response
- Lentil review- English grammar needs to be carefully checked by authors, examples:
Reply: Grammar corrections have been checked.
- Line 68 “…for the required trait(s)…”
Correction made: Necessary correction have been incorporated.
- Line 105 “…stored in a clean container…”
Correction made: Necessary correction have been incorporated.
- Lines 88-98: The study has a major weakness as they are reported the results of one year using 3 environments. This is a preliminary study of yield and seed mineral nutrient concentration stability across environments.
Reply: Stability analysis can be performed using data obtained across several locations for one year, or several years at one location, or several locations for several years. All the three serve the purpose and results are comparable.
- Lines: 102-111: The authors are describing the determination of seed mineral nutrient concentrations, not seed content, based the description of grinding 3 grams of grains for the each of the 96 samples and the reported mg/kg. The text should be changed to concentration throughout the manuscript.
Content describes per seed. This could be calculated
Correction made: The word ‘’Content’’ has been replaced by ‘’concentration’’ throughout the manuscript.
Reviewer 2 Report
I have attached a version of the manuscript with numerous grammatical revisions. The authors are encouraged to accept the majority of these revisions to improve the quality of English narrative.
Although the scope of the study was limited in terms of years (one year), it did include 96 genotypes, which was a robust sampling of lentil diversity compared to many yield trials used for GGE/AMMI analysis. The study only considered two minerals, iron and zinc, but these are undoubtedly the most important mineral nutrients in terms of global incidence of dietary deficiencies. The authors were able to identify several lentil genotypes that stably produced seed with high concentrations of iron or zinc and these materials will be useful for breeding programs and genetic studies.
Below are specific comments that should be addressed before the manuscript is accepted for publication.
- Please include the following citations in the manuscript as they reflect robust efforts by scientists in Canada and the USA to examine mineral concentrations in lentil. Ray et al. 2014. Crop Sci. 54:1698-1708, Vandemark et al. 2018. Crop J. 6:253-262.
- Line 99. How big were the plots? Please include the number of rows per plot and the length of each row, which will allow the reader to estimate the number of plants per plot.
- Line 188-189. Please describe in greater detail the variables GEI signal and GEI noise and how they are calculated.
- Line 197. Please double check that you meant to reference Table 3. I do not see how information presented in Table 3 supports this statement about no residual.
Comments for author File: Comments.pdf
Author Response
I have attached a version of the manuscript with numerous grammatical revisions. The authors are encouraged to accept the majority of these revisions to improve the quality of English narrative.
Reply: Necessary corrections have been incorporated.
Although the scope of the study was limited in terms of years (one year), it did include 96 genotypes, which was a robust sampling of lentil diversity compared to many yield trials used for GGE/AMMI analysis. The study only considered two minerals, iron and zinc, but these are undoubtedly the most important mineral nutrients in terms of global incidence of dietary deficiencies. The authors were able to identify several lentil genotypes that stably produced seed with high concentrations of iron or zinc and these materials will be useful for breeding programs and genetic studies.
We would like to thank you for your critical suggestions and comments.
Below are specific comments that should be addressed before the manuscript is accepted for publication.
- Please include the following citations in the manuscript as they reflect robust efforts by scientists in Canada and the USA to examine mineral concentrations in lentil. Ray et al. 2014. Crop Sci. 54:1698-1708, Vandemark et al. 2018. Crop J. 6:253-262.
Included in the manuscript:
1) Ray, H.; Bett, K.; Tar’an, B.; Vandenberg, A.; Thavarajah, D.; Warkentin, T.D. Mineral micronutrient content of cultivars of field pea, chickpea, common bean, and lentil grown in Saskatchewan, Canada. Crop Sci.2014, 54, 1698-1708.
2) Vandemark, G.J.; Grusak, M.A.; McGee, R.J. Mineral concentrations of chickpea and lentil cultivars and breeding lines grown in the U.S. Pacific Northwest. Crop J.2018, 6, 253-262.
- Line 99. How big were the plots? Please include the number of rows per plot and the length of each row, which will allow the reader to estimate the number of plants per plot.
Reply: Entries were planted in randomized block design in three rows of five meter length with two replications and inter-row spacing of 25 cm; plant to plant spacing of 5 cm.
- Line 188-189. Please describe in greater detail the variables GEI signal and GEI noise and how they are calculated.
Reply: All the detail has been described in manuscript.
- Line 197. Please double check that you meant to reference Table 3. I do not see how information presented in Table 3 supports this statement about no residual.
Reply: (Table 3), written in line 197 has been omitted out.
Author Response File: Author Response.pdf
