Transfer of Durable Stripe Rust Resistance Gene Yr39 into Four Chinese Elite Wheat Cultivars Using Marker-Assisted Selection
, Zhensheng Kang 2, Xianming Chen 3, Xinli Zhou 1,* and Suizhuang Yang 1,*
Round 1
Reviewer 1 Report
The manuscript describes the use of markers linked to the Yr39 resistance gene to monitor the transfer of resistance into elite germplasm. Agronomic traits are also evaluated to identify potential yield losses due to the introduction of the resistance gene.
Overall, the manuscript is comprehensible, the results are sound, and the conclusions are based on the results. However, from the text, I understand that the BC1F4 were tested for resistance in the field, and then the 71 selected lines were tested for agronomic traits. After that, the 71 selected BC1F6 lines were assessed for agronomic traits and Yr39 linked markers. The manuscript could expand on why field tests for resistance were done in the BC1F4 before the lines were tested using the markers. Presumably, the use of MAS is to increase breeding efficiency by selecting material by the use of markers before they are tested in the field.
In the discussion (lines 303-304), it is stated that “detection rate in the transfer population was not high, the repeatability was quite low”. However, this is not presented in the results, and this statement needs some further explanation.
Minor comments
Proof reading for language quality would improve the readability of the manuscript.
Line 48-48 “…due to the conservative of the primers,…” could be changed to ““…due to the conserved sequences of the primers,…” or similar.
Line 153 – replace “mark” with “marker” (2 instances).
Line 314 – Please reformulate the sentence “The result of the cost of resistance may be to maintain the polymorphism at this resistance site to be lost in other ways.”, as the meaning is not clear.
Figure 4 – ‘progeny lines’
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Reviewer 2 Report
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