Effect of Fomesafen on the Nutritional Quality and Amino Acids of Vigna angularis Based on Metabonomics
Round 1
Reviewer 1 Report (Previous Reviewer 1)
Dear editor and colleagues,
I have read the revised version of the “Effect of Fomesafen on Quality and Amino Acids of Vigna angularis Based on Metabonomics” manuscript resubmitted to agronomy-mdpi
The authors have provided the necessary technical explanations and I believe that data produced are valid. The manuscript also has been substantially improved in terms of English.
I have minor comments that the authors can take into consideration.
It is generally a good linguistic practice (across the text) when opening a sentence not to use number (1, 2, 3) but a letter (one, two, three etc).
Table 1 is not very informative and has too many details in digits. For instance, y=3.62534e 4x+2745.21904. In correlations studies it is advisable to indicate datapoints using figures and also add the equation. But since all statistics across AA are >0.99 (L155. The linearity R2 of all indicators is more significant than 0.99, indicating good linearity), it can go to supplementary data
Figure 1 needs a scale bar for comparison
Based on the above I recommend acceptance after minor revision
Author Response
Dear reviewer, thank you very much for your valuable suggestions for my Manuscript ID: agronomy-2154630. These suggestions have further improved the quality of my articles and are very helpful to my future writing. Thank you again. Now I will answer your suggestions:
Point 1: It is generally good linguistic practice (across the text) when opening a sentence not to use a number (1, 2, 3) but a letter (one, two, three, etc).
Response 1: Thank you very much for your suggestions. I agree with you very much, and make changes in the corresponding places of the full text, which are shown in red font.
Point 2: Table 1 is not very informative and has too many details in digits. For instance, y=3.62534e 4x+2745.21904. In correlation studies, it is advisable to indicate data points using figures and also add the equation. But since all statistics across AA are >0.99 (L155. The linearity R2 of all indicators is more significant than 0.99, indicating good linearity), it can go to supplementary data
Response 2: Thank you very much for your advice. I agree with you very much and added data in the corresponding position, which is displayed in red font.
Point 3: Figure 1 needs a scale bar for comparison.
Response 3: Thank you very much for your advice. I agree with you very much and added a scale in Figure 1.
Thank you again for helping me review my manuscript and giving me valuable suggestions.
Author Response File: 
Author Response.docx
Reviewer 2 Report (New Reviewer)
Review report for Agronomy manuscript Dec,28,2022
General comments: -
This study is very interesting and has a scientific topic with a great impact on the field. The manuscript will be suitable for publication after taking care of the following minor comments.
Detailed comments:
The English language and /writing style is fine needs some minor check spelling and grammar check
Abstract
_This section is missing the direct aim of the study. Please state the aim of the study clearly in this section.
Keywords:
-The keywords has been chosen very carefully and accurately but
- please add the word metabolomics to the keywords list
Introduction
-The introduction doesn’t provide sufficient background and it is missing enough relevant references
-This section needs to be elongated and enriched with more background about this topic.
Materials and Methods
-it is ok and adequate
Results:
The results are very interesting and well presented but some figures needs a better discussion (figure4and 7 and 10).
Figure 2 in line 201 please delete statistical diagram of
Also add description for the letters a,b,c,…. In the figure legends.
Discussion:
_This section is poorly written
- the author is strongly advised to combine the results and discussion in one section for better interpretation and discussion for the presented data.
Please rewrite and discuss in details, and fully discussed with related citations.
Conclusion
. This section is well written and the conclusion is supported by the results of this study and includes the most important findings.
References
This section is well written and UpToDate.
Author Response
Dear expert, thank you very much for your valuable suggestions for my Manuscript ID: agronomy-2154630. These suggestions have further improved the quality of my articles and are very helpful to my future writing. Thank you again. Now I will answer your suggestions:
Point 1: This section is missing the direct aim of the study. Please state the aim of the study clearly in this section.
Response 1: Thank you very much for your valuable suggestions. I agree with you very much and explained it in the summary, which is displayed in red font.
Point 2: The keywords have been chosen very carefully and accurately but please add the word metabolomics to the keywords list.
Response 2: Thank you very much for your valuable suggestions. I agree with you very much, and add the word metabonomics to the keyword section to display it in red font.
Point 3: The introduction doesn’t provide sufficient background and it is missing enough relevant references. This section needs to be elongated and enriched with more background about this topic.
Response 3: Thank you very much for your valuable suggestions. I agree with you very much, and make a supplement in the introduction, which is displayed in red font.
Point 4:The results are very interesting and well presented but some figures need a better discussion (figure4and 7 and 10). Figure 2 in line 201 please delete the statistical diagram Also add a description for the letters a,b,c,…. In the figure legends.
Response 4: Thank you very much for your valuable suggestions. I agree with you very much, and add it in the corresponding position. The supplementary content is displayed in red font.
Point 5:This section is poorly written the author is strongly advised to combine the results and discussion in one section for better interpretation and discussion of the presented data.
Please rewrite and discuss in detail, and fully discussed with related citations.
Response 5: Thank you very much for your valuable suggestions. I agree with you very much. Because the template of the Agronomy periodical clearly shows that the results and discussions are separated, my manuscript is written according to the template, but I have supplemented some contents in the discussion section, and the supplementary contents are displayed in red font.
Thank you again for helping me review my manuscript and giving me valuable suggestions.
Author Response File: Author Response.docx
Reviewer 3 Report (New Reviewer)
I would like to stress that I support the potential publication of this article due to its scientific interest. On the other hand, many aspects of the manuscript need to be extensively improved. I strongly advise the authors to take into serious consideration all of the following remarks to improve the quality of the presentation of their work.
Title: “Quality and Amino Acids”…….It would be better to mention the type of quality, e.g., nutritional quality, protein quality, etc.
Line 11-14…….“To explore the effect of………….chromatography-mass spectrometry (LC-MS)”….Revise the line….First, you need to mention a general problem and then the objective of the study. You should split this big line.
The abstract is not attractive. You need to highlight the key results. Revise…..“Through targeted amino acid analysis, 21 different amino acids…..” Vigna angularis is mentioned several times in the abstract…..You should mention the common name like red bean.
Line 39…..You should start the line like this Red bean (Vigna angularis)….is an important crop….Red bean (Vigna angularis)…..Earlier it was known as Xiaoshu and Chishu in China……In the rest lines, you should include common name (Red bean or adzuki bean) only and try to avoid the scientific name in the text.
Its economic value ranks first among all cereals. Can you present some data or references??
I think the Introduction part can be made more interesting with recent and relevant citations. You can take help from these articles https://doi.org/10.1016/j.scienta.2021.110075; https://doi.org/10.3390/land11040536
Line 68….LC-MS technology mentioned for the first time in Text…Therefore, elaborate LC-MS.
Line 147-151….Check spacing, typos…..10mg… 10 mg……0.1mol/ L HCl…0.1 mol/L HCl……20umol / mL…20 umol/mL…….25μL, added 90 %... 25 μL, added 90%......Check such typos throughout the article.
Table 1…..The values in the equation can be rounded to two decimal places. Follow the same in other Tables.
The preparation of extracts and LC-MS detection methods can be improved.
Line 165 and 172…..p<0.05….P<0.05…..Maintain similarity throughout the manuscript.
Include the version of Statistical Software used.
Figure 2…..Mention about letters a, b (shown above the bar) in the footnote. The X and Y-axis title…Content and Grouping…..Check the font and Use of Capital letters.
Line 357-386…..Producing correlation and heat map is vague. If both needs to be presented, explain in detail. This section is not described well, the use of both correlation techniques.
As there is noteworthy and voluminous data, I suggest the discussion can be elaborated with sub-sections. The discussion section must be enriched.
Line 455….According to P<0.05, Impact ≥0.001, five metabolic………Revise the statement.
Conclusion: What health products is suggested from the study as mentioned in the objective (Line 72)?
References: Improve the literature.
Overall, I feel the flow of writing and English needs a lot of improvement. If you take help from English Editing and other services, it will be better.
Author Response
Dear expert, thank you very much for your valuable suggestions for my Manuscript ID: agronomy-2154630. These suggestions have further improved the quality of my articles and are very helpful to my future writing. Thank you again. Now I will answer your suggestions:
Point 1: Title: “Quality and Amino Acids”…….It would be better to mention the type of quality, e.g., nutritional quality, protein quality, etc.
Response 1: Thank you very much for your valuable suggestions. I agree with you very much and add "nutritional quality" in the title, which is displayed in red font.
Point 2: Line 11-14…….“To explore the effect of………….chromatography-mass spectrometry (LC-MS)”….Revise the line….First, you need to mention a general problem and then the objective of the study. You should split this big line.
Response 2: Thank you very much for your valuable suggestions. I agree with you very much and modify it in the corresponding position in the text. The modified content is displayed in red font.
Point 3: The abstract is not attractive. You need to highlight the key results. Revise…..“Through targeted amino acid analysis, 21 different amino acids…..” Vigna angularis is mentioned several times in the abstract…..You should mention the common name like red bean.
Response 3: Thank you very much for your valuable suggestions. I agree with you very much but I use Vigna angularis instead of red bean in the manuscript to maintain the consistency of the names of test materials in the article.
Point 4: Line 39…..You should start the line like this Red bean (Vigna angularis)….is an important crop….Red bean (Vigna angularis)…..Earlier it was known as Xiaoshu and Chishu in China……In the rest lines, you should include common names (Red bean or adzuki bean) only and try to avoid the scientific name in the text.
Response 4: Thank you very much for your valuable suggestions. I agree with you very much, and modify it in the corresponding position in the text. The modified content is displayed in red font.
Point 5: Its economic value ranks first among all cereals. Can you present some data or references??
Response 5: Thank you very much for your valuable suggestions. I agree with you very much and add the corresponding position in the text. The supplementary content will be displayed in red font.
Point 6: I think the Introduction part can be made more interesting with recent and relevant citations. You can take help from these articles https://doi.org/10.1016/j.scienta.2021.110075; https://doi.org/10.3390/land11040536
Response 6: Thank you very much for your valuable suggestions. I agree with you very much. After I carefully read the two articles you recommended, I found that they are very helpful for my team's future research, but I'm sorry that I can't use them as a part of my manuscript in this article. I believe that they will be quoted in future articles, which will enrich my research background.
Point 7: Line 68….LC-MS technology mentioned for the first time in Text…Therefore, elaborate LC-MS.
Response 7: Thank you very much for your valuable suggestions. I agree with you very much, and modify it in the corresponding position in the text. The modified content is displayed in red font.
Point 8: Line 147-151….Check spacing, typos…..10mg…10 mg……0.1mol/ L HCl…0.1 mol/L HCl……20umol/mL…20 umol/mL…….25μL, added 90 %...25 μL, added 90%......Check such typos throughout the article.
Response 8: Thank you very much for your valuable suggestions. I agree with you very much and check and revise it in the corresponding position of the full text.
Point 9: Table 1…..The values in the equation can be rounded to two decimal places. Follow the same in other Tables.
Response 9: Thank you very much for your valuable suggestions. I agree with you very much and check and revise it in the corresponding position of the full text. The modified content is displayed in red font.
Point 10: The preparation of extracts and LC-MS detection methods can be improved.
Response 10: Thank you very much for your valuable suggestions. I agree with you very much, however, the innovation of my current research is not to optimize the preparation of extracts and develop new LC-MS detection methods. Later research will consider improving the preparation of extract and the detection method of LC-MS.
Point 11: Line 165 and 172…..p<0.05….P<0.05…..Maintain similarity throughout the manuscript.
Response 11: Thank you very much for your valuable suggestions. I agree with you very much, and modify it in the corresponding position in the text. The modified content is displayed in red font.
Point 12: Include the version of Statistical Software used.
Response 12: Thank you very much for your valuable suggestions. I agree with you very much, however, the version of statistical software shown in my manuscript is the version I used when processing data.
Point 13: Figure 2…..Mention letters a and b (shown above the bar) in the footnote. The X and Y-axis title…Content and Grouping…..Check the font and Use of Capital letters.
Response 13: Thank you very much for your valuable suggestions. I agree with you very much and check and revise it in the corresponding position of the full text. The modified content is displayed in red font.
Point 14: Line 357-386…..Producing correlation and heat maps is vague. If both needs to be presented, explain in detail. This section is not described well, the use of both correlation techniques.
Response 14: Thank you very much for your valuable suggestions. I agree with you very much, and modify it in the corresponding position in the text. The modified content is displayed in red font.
Point 15: As there is noteworthy and voluminous data, I suggest the discussion can be elaborated with sub-sections. The discussion section must be enriched.
Response 15: Thank you very much for your valuable suggestions. I agree with you very much and add the corresponding position in the text. The supplementary content will be displayed in red font.
Point 16: Line 455….According to P<0.05, Impact ≥0.001, five metabolic………Revise the statement.
Response 16: Thank you very much for your valuable suggestions. I agree with you very much, and modify it in the corresponding position in the text. The modified content is displayed in red font.
Point 17: Conclusion: What health products are suggested from the study as mentioned in the objective (Line 72)?
Response 17: Thank you very much for your valuable suggestions. I agree with you very much and add the corresponding position in the text. The supplementary content will be displayed in red font.
Point 18: References: Improve the literature.
Response 18: Thank you very much for your valuable suggestions. I agree with you very much and add the corresponding position in the text. The supplementary content will be displayed in red font.
Thank you again for helping me review my manuscript and giving me valuable suggestions.
Author Response File: Author Response.docx
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
Round 1
Reviewer 1 Report
Dear editor and colleagues,
I have read the revised version of the “Effect of Fomesafen on Quality and Amino Acids of Vigna angularis Based on Metabonomics” manuscript resubmitted to agronomy-mdpi
The authors have provided the necessary technical explanations and I believe that data produced are valid. The manuscript also has been substantially improved in terms of English.
I have minor comments that the authors can take into consideration.
It is generally a good linguistic practice (across the text) when opening a sentence not to use number (1, 2, 3) but a letter (one, two, three etc).
Table 1 is not very informative and has too many details in digits. For instance, y=3.62534e 4x+2745.21904. In correlations studies it is advisable to indicate datapoints using figures and also add the equation. But since all statistics across AA are >0.99 (L155. The linearity R2 of all indicators is more significant than 0.99, indicating good linearity), it can go to supplementary data
Figure 1 needs a scale bar for comparison
Based on the above I recommend acceptance after minor revision
