Research on a Biofilter for a Typical Application Scenario in China: Treatment of Pesticide Residue Wastewater in Orchards
Abstract
:1. Introduction
2. Materials and Methods
2.1. Description of the Experimental Site
2.2. Preparation of the Biomixture
2.3. Design of Pilot-Scale Biofilter System
2.4. Water and Biomixture Analyses
2.5. Microbial Sampling and Measurements
2.6. Statistical Analysis
3. Results and Discussion
3.1. Pesticide Degradation and Biofiltration Performance
3.2. Analysis of Microbial Community at the OTU Level
3.3. Analysis of Microbial Community Taxonomy
3.3.1. Identification of Dominant Microbes
3.3.2. Relative Abundance Dynamics of Dominant and Subdominant Microbes
3.3.3. Cluster Analysis for Similarity of Samples Collected in Different Phases
3.4. Relationship between Imidacloprid Concentration and Microbial Community Structure
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Shannon | Simpson | Ace | Chao | Coverage |
---|---|---|---|---|---|
A1 | 1.16 | 0.39 | 25.90 | 25.20 | 0.99 |
A2 | 1.70 | 0.23 | 23.49 | 23.00 | 0.99 |
A3 | 1.64 | 0.25 | 33.71 | 31.75 | 0.99 |
A4 | 1.64 | 0.28 | 26.30 | 26.00 | 0.99 |
A5 | 1.88 | 0.22 | 29.42 | 28.50 | 0.99 |
1.60 | 0.27 | 27.76 | 26.89 | 0.99 | |
S | 0.27 | 0.069 | 3.94 | 3.35 | 0 |
Phylum | Average Relative Abundance |
---|---|
Firmicutes | 29.45% |
Actinobacteriota | 25.21% |
Proteobacteria | 24.92% |
Bacteroidota | 6.87% |
Chloroflexi | 4.97% |
Patescibacteria | 3.45% |
Gemmatimonadota | 1.26% |
Acidobacteriota | 0.79% |
Myxococcota | 0. 75% |
Verrucomicrobiota | 0.71% |
Genus | Average Relative Abundance |
---|---|
Bacillus | 19.27% |
Methylobacter | 9.06% |
unclassified_f__Microbacteriaceae | 5.46% |
Streptomyces | 3.97% |
Hydrogenispora | 3.50% |
Methylocystis | 2.54% |
Arthrobacter | 2.31% |
Nocardioides | 2.01% |
norank_f__JG30-KF-CM45 | 2.01% |
Ciceribacter | 1.74% |
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Zeng, J.; Yuan, Q.; Xu, W.; Li, H.; Li, M.; Lei, X.; Wang, W.; Lin, Q.; Li, X.; Xu, R.; et al. Research on a Biofilter for a Typical Application Scenario in China: Treatment of Pesticide Residue Wastewater in Orchards. Agronomy 2024, 14, 934. https://doi.org/10.3390/agronomy14050934
Zeng J, Yuan Q, Xu W, Li H, Li M, Lei X, Wang W, Lin Q, Li X, Xu R, et al. Research on a Biofilter for a Typical Application Scenario in China: Treatment of Pesticide Residue Wastewater in Orchards. Agronomy. 2024; 14(5):934. https://doi.org/10.3390/agronomy14050934
Chicago/Turabian StyleZeng, Jin, Quanchun Yuan, Wenzhi Xu, Hailong Li, Menghui Li, Xiaohui Lei, Wei Wang, Qiang Lin, Xue Li, Rui Xu, and et al. 2024. "Research on a Biofilter for a Typical Application Scenario in China: Treatment of Pesticide Residue Wastewater in Orchards" Agronomy 14, no. 5: 934. https://doi.org/10.3390/agronomy14050934