Identification and Molecular Characterization of a 16SrII-A Phytoplasma Associated with Cucumber Phyllody in China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Molecular Identification
2.2. Sequence Analysis
3. Results
3.1. Field Survey and Molecular Detection
3.2. Phylogenetic Analysis
4. Discussion
4.1. Phytoplasma Disease and Molecular Detection
4.2. Phytoplasmas in China
4.3. Prospects
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Strain | Associated Plant Disease and Origin | 16S rDNA Group/Subgroup | GenBank Accession No. |
---|---|---|---|
CuV1 | cucumber virescence; Taiwan | 16SrI-X | GU361755 |
CuPh | cucumber phyllody; India | 16SrI-X | MZ401356 |
CuPh | cucumber phyllody; Iran | 16SrII-D | KR822804 |
CuPh | cucumber phyllody; Iran | 16SrII-D | JN574839 |
CUP-Bn | cucumber phyllody; Iran | 16SrII-D | MK453519 |
CuPh | cucumber phyllody; Iran | 16SrVI-A | JF508516 |
CuPh | cucumber phyllody; Iran | 16SrXII-A | MK402983 |
EgPh | eggplant phyllody; China | 16SrII-A | MH667642 |
DeoWB | Desmodium ovalifolium witches’ broom; China | 16SrII-A | GU113152 |
PnWB | peanut witches’ broom; Taiwan | 16SrII-A | L33765 |
GvWB-YNym | Gendarussa vulgaris witches’ broom; China | 16SrII-A | MN535183 |
WBDL | witches’ broom disease of lime; Oman | 16SrII-B | U15442 |
CaWBYNO1 | cactus witches’ broom; China | 16SrII-C | AJ293216 |
PpYC | papaya yellow crinkle; Australia | 16SrII-D | Y10097 |
PEP | Picris echioides phyllody; Italy | 16SrII-E | Y16393 |
CaWBYN11 | cactus witches’ broom; China | 16SrII-F | EU099556 |
PpBT | papaya bunchy top; Cuba | 16SrII-N | JF781309 |
PX11Ct1 | Western-X disease; USA | 16SrIII-A | JQ044393 |
CYE-Or | clover yellow edge; USA | 16SrIII-B | AF189288 |
GRY-GR1 | goldenrod yellowing; USA | 16SrIII-D | FJ376627 |
LYJ-C8 | coconut lethal yellowing; USA | 16SrIV-A | AF498307 |
EY1 | elm yellowing; USA | 16SrV-A | AY197655 |
JWB-G1 | jujube witches’ broom; Japan | 16SrV-B | AB052876 |
CP | clover proliferation; Canada | 16SrVI-A | AY390261 |
AshY1 | ash yellowing; USA | 16SrVII-A | AF092209 |
LfWB | loofah witches’ broom; Taiwan | 16SrVIII-A | AF248956 |
ESFY-G1 | European stone fruit yellowing; Germany | 16SrX-F | AJ542544 |
RYD-Th | rice yellow dwarf; Thailand | 16SrXI-A | AB052873 |
HibWB26 | hibiscus witches’ broom; Brazil | 16SrXV-A | AF147708 |
GWB#4 | ‘Guazuma ulmifolia’ witches’ broom; Costa Rica | 16SrXV-B | HQ258882 |
APPT10-NE | American potato purple top wilt; USA | 16SrXVIII-B | DQ174121 |
Pin127s | pine shoot proliferation; Spain | 16SrXXI-A | AJ632155 |
BVGY | Buckland valley grapevine yellowing; Australia | 16SrXXIII-A | AY083605 |
IM-1 | Cassia italica witches’ broom; Oman | 16SrXXIX-A | EF666051 |
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Xi, Y.; Du, M.; Tang, Y.; She, X.; Lan, G.; Yu, L.; Ding, S.; He, Z.; Li, Z. Identification and Molecular Characterization of a 16SrII-A Phytoplasma Associated with Cucumber Phyllody in China. Agronomy 2024, 14, 1873. https://doi.org/10.3390/agronomy14081873
Xi Y, Du M, Tang Y, She X, Lan G, Yu L, Ding S, He Z, Li Z. Identification and Molecular Characterization of a 16SrII-A Phytoplasma Associated with Cucumber Phyllody in China. Agronomy. 2024; 14(8):1873. https://doi.org/10.3390/agronomy14081873
Chicago/Turabian StyleXi, Youwei, Mengdan Du, Yafei Tang, Xiaoman She, Guobing Lan, Lin Yu, Shanwen Ding, Zifu He, and Zhenggang Li. 2024. "Identification and Molecular Characterization of a 16SrII-A Phytoplasma Associated with Cucumber Phyllody in China" Agronomy 14, no. 8: 1873. https://doi.org/10.3390/agronomy14081873