Characterization of Strubbelig-Receptor Family (SRF) Related to Drought and Heat Stress Tolerance in Upland Cotton (Gossypium hirsutum L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sequence Retrieval of SRF Protein Family Members
2.2. Construction of Phylostratum, and Phylogeny Tree
2.3. Sequence Logos and Conserved Amino Acid Analysis
2.4. Promoter, Protein Motifs, and Gene Structure Analyses
2.5. Sub-Cellular Localization, Gene Mapping on Chromosomes, Gene Duplication, Collinearity and Ratios of Ka/Ks
2.6. In Silico Expression Analysis of GhSRF Genes
2.7. Expression Analysis and Validation of Candidate Genes
3. Results
3.1. Identification and Physiochemical Properties of SRF Genes in Cotton
3.2. Sequence Alignment, Phylostratum, and Phylogenetic Tree
3.3. Finding of Conserved Amino Acid Patterns
3.4. Analysis of Cis-Element in Promoter, Motif Finding and Gene Structure
3.5. Gene Mapping on Chromosomes, Ratio of Ka/Ks, Gene Duplication, and Sub-Cellular Localization
3.6. Gene Expression Analysis of GhSRF Genes Under Drought and Heat Stress Conditions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ahmad, F.; Rehman, S.U.; Rahman, M.H.U.; Ahmad, S.; Khan, Z. Characterization of Strubbelig-Receptor Family (SRF) Related to Drought and Heat Stress Tolerance in Upland Cotton (Gossypium hirsutum L.). Agronomy 2024, 14, 1933. https://doi.org/10.3390/agronomy14091933
Ahmad F, Rehman SU, Rahman MHU, Ahmad S, Khan Z. Characterization of Strubbelig-Receptor Family (SRF) Related to Drought and Heat Stress Tolerance in Upland Cotton (Gossypium hirsutum L.). Agronomy. 2024; 14(9):1933. https://doi.org/10.3390/agronomy14091933
Chicago/Turabian StyleAhmad, Furqan, Shoaib Ur Rehman, Muhammad Habib Ur Rahman, Saghir Ahmad, and Zulqurnain Khan. 2024. "Characterization of Strubbelig-Receptor Family (SRF) Related to Drought and Heat Stress Tolerance in Upland Cotton (Gossypium hirsutum L.)" Agronomy 14, no. 9: 1933. https://doi.org/10.3390/agronomy14091933