Multi-Omics Analysis Reveals the Molecular Mechanisms of the Glycolysis and TCA Cycle Pathways in Rhododendron chrysanthum Pall. under UV-B Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Treatment of Plant Material
2.2. Determination of Chlorophyll Fluorescence Parameters of R. chrysanthum
2.3. Determination of the R. chrysanthum Transcriptome
2.3.1. RNA Extraction and Sequencing
2.3.2. Filtering, Reassembly, and Annotation of Data
2.3.3. Differential Gene Expression Analysis
2.4. Determination of R. chrysanthum’s Whole Protein Profile Using 4D Label-Free Quantitative Proteomics
2.4.1. Protein Extraction
2.4.2. Trypsin Digestion
2.4.3. Mass Spectrometer
2.4.4. Database Search
2.4.5. Differential Proteins Expression Analysis
2.5. Annotation Methods
2.5.1. KEGG Pathway Annotation
2.5.2. Subcellular Localization
2.6. Widely Targeted Metabolome Testing
2.6.1. Extraction
2.6.2. Chromatography and Mass Spectrometry Acquisition Conditions
2.7. Analysis of Non-Covalent Interactions of Proteins
2.8. Data Processing
3. Results
3.1. Inhibition of R. chrysanthum Photosynthesis and Consequent Effects on Carbon Metabolism under UV-B Stress
3.2. DEGs Enrichment in Glycolysis and TCA Cycling Pathways in R. chrysanthum under UV-B Stress
3.3. R. chrysanthum under UV-B Stress Showed a Large Number of DEPs
3.4. UV-B Stress Leads to Differential Expression of Important Proteins in TCA and Glycolytic Pathways
3.5. Changes in Expression of DMs in Glycolysis and TCA Cycling Pathways of R. chrysanthum under UV-B Stress
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yu, W.; Gong, F.; Cao, K.; Zhou, X.; Xu, H. Multi-Omics Analysis Reveals the Molecular Mechanisms of the Glycolysis and TCA Cycle Pathways in Rhododendron chrysanthum Pall. under UV-B Stress. Agronomy 2024, 14, 1996. https://doi.org/10.3390/agronomy14091996
Yu W, Gong F, Cao K, Zhou X, Xu H. Multi-Omics Analysis Reveals the Molecular Mechanisms of the Glycolysis and TCA Cycle Pathways in Rhododendron chrysanthum Pall. under UV-B Stress. Agronomy. 2024; 14(9):1996. https://doi.org/10.3390/agronomy14091996
Chicago/Turabian StyleYu, Wang, Fushuai Gong, Kun Cao, Xiaofu Zhou, and Hongwei Xu. 2024. "Multi-Omics Analysis Reveals the Molecular Mechanisms of the Glycolysis and TCA Cycle Pathways in Rhododendron chrysanthum Pall. under UV-B Stress" Agronomy 14, no. 9: 1996. https://doi.org/10.3390/agronomy14091996