EZH2 and Endometrial Cancer Development: Insights from a Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Ethics
2.2. Genotyping and DNA Recombination Analysis
2.3. Histology, Immunohistochemistry, and Immunofluorescence
2.4. Western Blot
2.5. Hypoxia Staining
2.6. Enzyme-Linked Immunosorbent Assay (ELISA)
2.7. Hormone Assays
2.8. Quantitative Reverse Transcription-PCR
2.9. Statistical Analysis
3. Results
3.1. Generation of Mice with Conditional Deletion of Ezh2 and Pten
3.2. Loss of EZH2 Reduces Tumor Burden during Early Carcinogenesis but Negatively Impacts Disease Outcome
3.3. Ezh2 and Pten Deletion Enhances the Accumulation of Intraluminal Neutrophils Compared with Pten Deletion Alone
3.4. Factors Contributing to the Developmental Trajectory of Endometrial Cancer Lacking PTEN and EZH2
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Manufacturer | Cat. # | Species | IHC/IF | WB |
---|---|---|---|---|---|
EZH2 | Cell Signaling (Danvers, MA, USA) | 5246 | Rabbit | 1:400 | 1:1000 |
pAKT | Cell Signaling | 4060 | Rabbit | 1:50 | 1:2000 |
Ki67 | Cell Signaling | 12202 | Rabbit | 1:500 | |
ECAD | Cell Signaling | 3195 | Rabbit | 1:400 | |
KRT14 | Thermo Fisher Scientific | PA5-16722 | Rabbit | 1:400 | |
ΔNp63 | BioLegend (San Diego, CA, USA) | 619001 | Rabbit | 1:200 | |
F4/80 | Cell Signaling | 70076 | Rabbit | 1:250 | |
LY6G | BioLegend | 127601 | Rat | 1:500 | |
PTEN | Cell Signaling | 9188 | Rabbit | 1:1000 | |
AKT | Cell Signaling | 4691 | Rabbit | 1:1000 | |
H3K27me3 | Cell Signaling | 9733 | Rabbit | 1:1000 | |
Histone H3 | Cell Signaling | 4499 | Rabbit | 1:5000 | |
ACTB | MilliporeSigma | A3854 | Mouse | 1:50,000 |
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Fang, X.; Ni, N.; Wang, X.; Tian, Y.; Ivanov, I.; Rijnkels, M.; Bayless, K.J.; Lydon, J.P.; Li, Q. EZH2 and Endometrial Cancer Development: Insights from a Mouse Model. Cells 2022, 11, 909. https://doi.org/10.3390/cells11050909
Fang X, Ni N, Wang X, Tian Y, Ivanov I, Rijnkels M, Bayless KJ, Lydon JP, Li Q. EZH2 and Endometrial Cancer Development: Insights from a Mouse Model. Cells. 2022; 11(5):909. https://doi.org/10.3390/cells11050909
Chicago/Turabian StyleFang, Xin, Nan Ni, Xiaofang Wang, Yanan Tian, Ivan Ivanov, Monique Rijnkels, Kayla J. Bayless, John P. Lydon, and Qinglei Li. 2022. "EZH2 and Endometrial Cancer Development: Insights from a Mouse Model" Cells 11, no. 5: 909. https://doi.org/10.3390/cells11050909