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Article

CTRP13-Mediated Effects on Endothelial Cell Function and Their Potential Role in Obesity

1
Experimental Cardiology, Department of Internal Medicine I, Justus Liebig University Giessen, 35390 Giessen, Germany
2
Institute of Physiology, Justus Liebig University Giessen, 35390 Giessen, Germany
3
Department of Cardiovascular Surgery Giessen, University-Hospital Giessen and Marburg, Justus Liebig University Giessen, 35390 Giessen, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2024, 13(15), 1291; https://doi.org/10.3390/cells13151291 (registering DOI)
Submission received: 6 May 2024 / Revised: 25 July 2024 / Accepted: 29 July 2024 / Published: 31 July 2024

Abstract

Background: Obesity, a major component of cardiometabolic syndrome, contributes to the imbalance between pro- and anti-atherosclerotic factors via dysregulation of adipocytokine secretion. Among these adipocytokines, the C1q/TNF-related proteins (CTRPs) play a role in the modulation of atherosclerosis development and progression. Here, we investigated the vascular effects of CTRP13. Results: CTRP13 is not only expressed in adipose tissue but also in vessels/endothelial cells (ECs) of mice, rats, and humans. Obese individuals (mice, rats, and humans) showed higher vascular CTRP13 expression. Human Umbilical Vein Endothelial Cells (HUVECs), cultured in the presence of serum from obese mice, mimicked this obesity-associated effect on CTRP13 protein expression. Similarly, high glucose conditions and TNF-alpha, but not insulin, resulted in a strong increase in CTRP13 in these cells. Recombinant CTRP13 induced a reduction in EC proliferation via AMPK. In addition, CTRP13 reduced cell cycle progression and increased p53 phosphorylation and p21 protein expression, but reduced Rb phosphorylation, with the effects largely depending on alpha-2 AMPK as suggested by adenoviral overexpression of dominant-negative (DN) or wild-type (WT) alpha 1/alpha 2 AMPK. Conclusion: The present study demonstrates that CTRP13 expression is induced in ECs under diabetic conditions and that CTRP13 possesses significant vaso-modulatory properties which may have an impact on vascular disease progression in patients.
Keywords: adipokines; endothelium; AMPK; proliferation; cell cycle; obesity adipokines; endothelium; AMPK; proliferation; cell cycle; obesity

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MDPI and ACS Style

Aslam, M.; Li, L.; Nürnberger, S.; Niemann, B.; Rohrbach, S. CTRP13-Mediated Effects on Endothelial Cell Function and Their Potential Role in Obesity. Cells 2024, 13, 1291. https://doi.org/10.3390/cells13151291

AMA Style

Aslam M, Li L, Nürnberger S, Niemann B, Rohrbach S. CTRP13-Mediated Effects on Endothelial Cell Function and Their Potential Role in Obesity. Cells. 2024; 13(15):1291. https://doi.org/10.3390/cells13151291

Chicago/Turabian Style

Aslam, Muhammad, Ling Li, Sina Nürnberger, Bernd Niemann, and Susanne Rohrbach. 2024. "CTRP13-Mediated Effects on Endothelial Cell Function and Their Potential Role in Obesity" Cells 13, no. 15: 1291. https://doi.org/10.3390/cells13151291

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