Mitofusin 2-Deficiency Suppresses Mycobacterium tuberculosis Survival in Macrophages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Mtb Culture and Infection
2.3. Western Blotting Analysis
2.4. Reagents
2.5. Transfection of Small Interfering RNA
2.6. Immunofluorescence
2.7. MFN2 Cloning
2.8. Apoptosis Assay
2.9. Oxygen Consumption Rate Assay
2.10. Statistical Analyses
3. Results
3.1. Mtb Infection Modulates Mitochondrial Function
3.2. MFN2 is Sensitive to the Survival of Attenuated Ra
3.3. Effect of MFN2 on Intracellular Survival of Mycobacteria
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lee, J.; Choi, J.-A.; Cho, S.-N.; Son, S.-H.; Song, C.-H. Mitofusin 2-Deficiency Suppresses Mycobacterium tuberculosis Survival in Macrophages. Cells 2019, 8, 1355. https://doi.org/10.3390/cells8111355
Lee J, Choi J-A, Cho S-N, Son S-H, Song C-H. Mitofusin 2-Deficiency Suppresses Mycobacterium tuberculosis Survival in Macrophages. Cells. 2019; 8(11):1355. https://doi.org/10.3390/cells8111355
Chicago/Turabian StyleLee, Junghwan, Ji-Ae Choi, Soo-Na Cho, Sang-Hun Son, and Chang-Hwa Song. 2019. "Mitofusin 2-Deficiency Suppresses Mycobacterium tuberculosis Survival in Macrophages" Cells 8, no. 11: 1355. https://doi.org/10.3390/cells8111355