Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Extracellular Vesicle Isolation
2.3. Exosome Characterization by Nanoparticle Tracking Analysis (NTA)
2.4. Exosome Characterization by Transmission Electron Microscopy (TEM)
2.5. Western Blotting
2.6. MTT Assay
2.7. Collagen Expression Assay
2.8. Angiogenesis Assay
2.9. Bone Marrow-Derived Macrophage (BMDM) Isolation and Culture
2.10. Macrophage Polarization Assay
2.11. Mechanism Study
2.12. Mass Spectrometric Analysis of Exosomes
2.13. Bioinformatic Data Analysis
3. Results
3.1. Effects of Small Molecules on Exosomal Production Efficiency
3.2. Downregulation of Collagen Expression
3.3. Macrophage Polarization
3.4. Angiogenesis Effect
3.5. Effects of Small Molecule Modulators on Genes Affecting Exosome Biogenesis and Secretion
3.6. Effects of Small Molecule Modulators on Exosomal Protein Composition
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
MSC | mesenchymal stem cell |
FT | fenoterol |
NE | norepinephrine |
FK | foskolin |
MeDA | N-methyldopamine |
Mepn | mephenesin |
HCF | human cardiac fibroblast |
HUVEC | human umbilical vein endothelial cell |
BMDM | bone-marrow derived macrophage |
MVB | multi-vesicular body |
ESCRT | endosomal sorting complex required for transport |
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Wang, J.; Bonacquisti, E.E.; Brown, A.D.; Nguyen, J. Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes. Cells 2020, 9, 660. https://doi.org/10.3390/cells9030660
Wang J, Bonacquisti EE, Brown AD, Nguyen J. Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes. Cells. 2020; 9(3):660. https://doi.org/10.3390/cells9030660
Chicago/Turabian StyleWang, Jinli, Emily E. Bonacquisti, Adam D. Brown, and Juliane Nguyen. 2020. "Boosting the Biogenesis and Secretion of Mesenchymal Stem Cell-Derived Exosomes" Cells 9, no. 3: 660. https://doi.org/10.3390/cells9030660