A Public Mid-Density Genotyping Platform for Hexaploid Sweetpotato (Ipomoea batatas [L.] Lam)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Germplasm Selection and Whole-Genome Sequencing of a Sweetpotato Diversity Panel
2.2. SNP Discovery and Selection of 3K Marker Loci for Building a DArTag Genotyping Panel
2.3. Principles and Implementation of the DArTag Genotyping Assay
2.4. Validation of the Sweetpotato DArTag Panel
2.5. Genetic Map Construction
3. Results
3.1. The Sweetpotato 3K DArTag Genotyping Panel
3.2. Microhaplotypes from the Sweetpotato 3K DArTag Genotyping Panel
3.3. Panel Effectiveness in Extant Sweetpotato Accessions
3.4. Creation of a Linkage Map
4. Discussion
4.1. Assessment of DArTag Performance and Utility in a Complex Polyploid Genome
4.2. Improving the DArTag Panel as a Global Community
5. Conclusions
Access to and Limitations of the Sweetpotato 3K DArTag Panel
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, D.; Sandercock, A.M.; Mejia-Guerra, M.K.; Mollinari, M.; Heller-Uszynska, K.; Wadl, P.A.; Webster, S.A.; Beil, C.T.; Sheehan, M.J. A Public Mid-Density Genotyping Platform for Hexaploid Sweetpotato (Ipomoea batatas [L.] Lam). Genes 2024, 15, 1047. https://doi.org/10.3390/genes15081047
Zhao D, Sandercock AM, Mejia-Guerra MK, Mollinari M, Heller-Uszynska K, Wadl PA, Webster SA, Beil CT, Sheehan MJ. A Public Mid-Density Genotyping Platform for Hexaploid Sweetpotato (Ipomoea batatas [L.] Lam). Genes. 2024; 15(8):1047. https://doi.org/10.3390/genes15081047
Chicago/Turabian StyleZhao, Dongyan, Alexander M. Sandercock, Maria Katherine Mejia-Guerra, Marcelo Mollinari, Kasia Heller-Uszynska, Phillip A. Wadl, Seymour A. Webster, Craig T. Beil, and Moira J. Sheehan. 2024. "A Public Mid-Density Genotyping Platform for Hexaploid Sweetpotato (Ipomoea batatas [L.] Lam)" Genes 15, no. 8: 1047. https://doi.org/10.3390/genes15081047