Analysis of the 5′ Untranslated Region Length-Dependent Control of Gene Expression in Maize: A Case Study with the ZmLAZ1 Gene Family
Abstract
:1. Introduction
2. Materials and Methods
2.1. Genome-Wide Identification of the UTRs from Maize, Rice, and Arabidopsis
2.2. Gene Ontology (GO) Enrichment Analysis on UTR-Containing Genes
2.3. Sample Preparation
2.4. Gene 5′ UTR Cloning
2.5. GUS Histochemical Staining
2.6. GUS Activity Measurement
2.7. Analysis of Cis-Acting Elements
2.8. Statistical Analysis
3. Results and Discussion
3.1. Whole-Genome Survey of UTRs in Maize
3.2. Functional Enrichment Analysis of UTR-Containing Genes in Maize
3.3. Characterization of the 5′ UTR Region of the ZmLAZ1 Gene Family
3.4. Effect of 5′ UTR Length on Gene Expression
3.5. Cis-Elements Analysis of 5′ UTR of ZmLAZ1 Gene Family
3.6. The Potential Relationship between 5′ UTR Length and Gene Function in the LAZ1 Gene Family
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, B.; Liu, X.; Sun, M.; Sun, Y.; Liu, D.; Hao, L.; Tao, Y. Analysis of the 5′ Untranslated Region Length-Dependent Control of Gene Expression in Maize: A Case Study with the ZmLAZ1 Gene Family. Genes 2024, 15, 994. https://doi.org/10.3390/genes15080994
Liu B, Liu X, Sun M, Sun Y, Liu D, Hao L, Tao Y. Analysis of the 5′ Untranslated Region Length-Dependent Control of Gene Expression in Maize: A Case Study with the ZmLAZ1 Gene Family. Genes. 2024; 15(8):994. https://doi.org/10.3390/genes15080994
Chicago/Turabian StyleLiu, Bingliang, Xiaowei Liu, Min Sun, Yanxia Sun, Dayu Liu, Li Hao, and Yang Tao. 2024. "Analysis of the 5′ Untranslated Region Length-Dependent Control of Gene Expression in Maize: A Case Study with the ZmLAZ1 Gene Family" Genes 15, no. 8: 994. https://doi.org/10.3390/genes15080994