Genetic Evidence of SpGH9A3 in Leaf Morphology Variation of Spathiphyllum ‘Mojo’
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Morphological Traits of Leaves Measurement
2.3. Determination of Leaf Cellulose Content and Cellulase Activity
2.4. Analysis of Differential Gene and Quantitative Real-Time PCR Assays
2.5. Correlation Analysis
2.6. Sequence Analysis
2.7. Subcellular Localization
2.8. Phenotypic Analysis of Transgenic Plants
2.9. Statistical Analysis
3. Results
3.1. Comparison of the Leaf Morphologies of Spathiphyllum ‘Mojo’ (Wild) and ‘Mojo’-Ssm-1 (Mutant)
3.2. Comparison of Cellulose Content and Cellulase Activity
3.3. Screening of Differential Genes for Leaf Morphology
3.4. Bioinformatics Study of SpGH9A3 in Spathiphyllum
3.5. SpGH9A3 Protein Is Localized to the Cell Membrane
3.6. Overexpressing SpGH9A3 Changed the Leaf Morphology and Decreased Cellulose Content
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Stage | Number | Primary Growth and Morphological Characteristics |
---|---|---|
The curled leaf stage | W1/M1 | Approximately ten days after the leaf buds transition to white, the tips of the leaves gradually change from white to yellow/white, while the base of the leaves remains white. During this period, curled leaves develop, with the leaves being enveloped in leaf sheaths. |
The early spread stage | W2/M2 | Approximately 10 to 13 days after the formation of curled leaves, the leaves emerge from the leaf sheath and begin to gradually unfold. During this process, the leaf color transitions from yellow/white to a delicate green. |
The late spread stage | W3/M3 | Approximately ten days after the onset of leaf color change, the leaves transition from light green to a deeper green. During this period, the leaves gradually expand, completing their growth. At this stage, the characteristics of the leaves stabilize and exhibit no further changes. |
WT | OE of SpGH9A3 | SpGH9A3/WT (%) | |
---|---|---|---|
Leaf length (cm) | 1.36 ± 0.05 | 1.02 ± 0.10 * | 75.00% |
Leaf width (cm) | 0.49 ± 0.03 | 0.48 ± 0.05 | 97.96% |
Length–width ratio | 2.78 ± 0.19 | 2.14 ± 0.28 * | 76.98% |
Petiole length (cm) | 0.57 ± 0.03 | 0.69 ± 0.04 * | 121.05% |
Leaf area (cm2) | 0.42 ± 0.03 | 0.33 ± 0.05 * | 78.57% |
Silique length (cm) | 1.25 ± 0.06 | 0.86 ± 0.06 * | 68.80% |
Plant height (cm) | 25.49 ± 1.42 | 17.63 ± 1.00 * | 69.16% |
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Yang, S.; Hu, M.; Wu, R.; Hou, Z.; Zhang, H.; He, W.; Gao, L.; Liao, F. Genetic Evidence of SpGH9A3 in Leaf Morphology Variation of Spathiphyllum ‘Mojo’. Genes 2024, 15, 1132. https://doi.org/10.3390/genes15091132
Yang S, Hu M, Wu R, Hou Z, Zhang H, He W, Gao L, Liao F. Genetic Evidence of SpGH9A3 in Leaf Morphology Variation of Spathiphyllum ‘Mojo’. Genes. 2024; 15(9):1132. https://doi.org/10.3390/genes15091132
Chicago/Turabian StyleYang, Songlin, Minghua Hu, Runxin Wu, Zhiwen Hou, Huan Zhang, Wenying He, Lili Gao, and Feixiong Liao. 2024. "Genetic Evidence of SpGH9A3 in Leaf Morphology Variation of Spathiphyllum ‘Mojo’" Genes 15, no. 9: 1132. https://doi.org/10.3390/genes15091132