Identification of Key Proteins Related to Cashmere Fiber Diameter by Integrated Proteomics and Bioinformatic Analyses in the Alpas and Alxa Goat Breeds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Measurement of Important Economic Characteristics
2.3. Protein Extraction and Digestion
2.4. Label-Free LC/MS Quantitative and Qualitative and Profiling
2.5. Bioinformatics Analysis
2.6. Western Blotting
2.7. Co-Immunoprecipitation (Co-IP)
3. Results
3.1. Performance of Fiber Quality Traits
3.2. Proteome Analysis during Fiber Development
3.3. Proteome Analysis of Differentially Expressed Proteins during Fiber Development
3.4. Key Protein Structure Domain Analysis
3.5. Western Blot Analysis of KRT14 Expression
3.6. KRT14 Interacts with Other Proteins
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Number | Accession | Protein Name | Gene Name |
---|---|---|---|
1 | A0A452EME4 | Keratin 14 | KRT14 |
2 | A0A452FDX9 | IF rod domain-containing protein | LOC108633262 |
3 | A0A452FPA3 | KIAA1217 | KIAA1217 |
4 | A0A452E4U1 | Keratin 17 | KRT17 |
5 | A0A452FUI2 | IF rod domain-containing protein | KRT18 |
6 | A0A452G8R7 | Myosin heavy chain 2 | MYH2 |
7 | A0A452EZ21 | 26S proteasome non-ATPase regulatory subunit 1 | PSMD1 |
8 | A0A452G5G4 | Acid-sensing ion channel subunit 1 | ASIC1 |
9 | A0A452G188 | Proline-rich 11 | PRR11 |
10 | A0A5D6VZD1 | ABC-F family ATP-binding cassette domain-containing protein | FZ040_10900 |
11 | A0A452G6J7 | Obscurin, cytoskeletal calmodulin, and titin-interacting RhoGEF | OBSCN |
12 | A0A452FZK5 | LDL receptor-related protein 4 | LRP4 |
13 | A0A452ERA9 | Outer dynein arm-docking complex subunit 4 | ODAD4 |
14 | A0A5D6WAU5 | Leukotoxin LktA family filamentous adhesin | FZ040_03715 |
15 | A0A452E1K6 | G protein-coupled receptor 68 | GPR68 |
16 | A0A5D6WDH4 | DUF4405 domain-containing protein | FZ040_00610 |
17 | A0A452FNB0 | Glutamate-rich protein 1 | ERICH1 |
18 | A0A452ERJ7 | OMA1 zinc metallopeptidase | OMA1 |
19 | A0A452EE60 | SHC adaptor protein 1 | SHC1 |
20 | A0A452E7K6 | Serine/arginine repetitive matrix 2 | SRRM2 |
21 | A0A452FQE8 | MLLT1 super elongation complex subunit | MLLT1 |
22 | A0A452DL58 | FH2 domain-containing protein | N\A |
23 | A0A452FQU4 | Lengsin, lens protein with glutamine synthetase domain | LGSN |
24 | A0A452G661 | Mucin like 3 | MUCL3 |
25 | A0A452FWB4 | Corepressor interacting with RBPJ | CIR1 |
26 | A0A452ECY9 | Growth Differentiation Factor 5 | GDF5 |
27 | A0A452E2Z0 | Transmembrane protein 65 | TMEM65 |
28 | A0A5D6WHQ8 | Precorrin-4 C(11)-methyltransferase | cobM |
29 | A0A452G6A8 | Ceramide synthase 6 | CERS6 |
30 | A0A452E5L8 | Neurofibromin 1 | NF1 |
31 | A0A452F4W3 | Mov10 like RISC complex RNA helicase 1 | MOV10L1 |
32 | A0A452E392 | Protein LSM14 homolog B | LSM14B |
33 | A0A452DVQ0 | TOPBP1 interacting checkpoint and replication regulator | TICRR |
34 | A0A5D6WBZ4 | S41 family peptidase | FZ040_05185 |
35 | A0A452FL24 | Flagellum associated containing coiled-coil domains 1 | FLACC1 |
36 | A0A452G137 | Vitamin D-binding protein | GC |
37 | S4SAK0 | Pyruvate kinase | pklr |
38 | A0A452F3A5 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase | PLCB1 |
39 | A0A5D6VXX5 | Succinate--CoA ligase | sucC |
40 | A0A452ELG6 | Melan-A | MLANA |
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Zhang, C.; Qin, Q.; Wang, Y.; Wang, Z.; Liu, Z. Identification of Key Proteins Related to Cashmere Fiber Diameter by Integrated Proteomics and Bioinformatic Analyses in the Alpas and Alxa Goat Breeds. Genes 2024, 15, 1154. https://doi.org/10.3390/genes15091154
Zhang C, Qin Q, Wang Y, Wang Z, Liu Z. Identification of Key Proteins Related to Cashmere Fiber Diameter by Integrated Proteomics and Bioinformatic Analyses in the Alpas and Alxa Goat Breeds. Genes. 2024; 15(9):1154. https://doi.org/10.3390/genes15091154
Chicago/Turabian StyleZhang, Chongyan, Qing Qin, Yichuan Wang, Zhixin Wang, and Zhihong Liu. 2024. "Identification of Key Proteins Related to Cashmere Fiber Diameter by Integrated Proteomics and Bioinformatic Analyses in the Alpas and Alxa Goat Breeds" Genes 15, no. 9: 1154. https://doi.org/10.3390/genes15091154