Functional and Microbiological Responses of Iron–Carbon Galvanic Cell-Supported Autotrophic Denitrification to Organic Carbon Variation and Dissolved Oxygen Shaking
Abstract
:1. Introduction
2. Materials and Methods
2.1. Batch Experiments
2.2. Water Quality Analysis
2.3. DNA Extraction
2.4. High-Throughput Sequencing of 16S rRNA
2.5. Construction of nirS and cbbL Libraries in DB0
2.6. Quantitative PCR of 16S rRNA, nirS, and cbbL
3. Results
3.1. Influence of OC and DO on the Performance of Fe(0)/C-AND
3.2. Community Structure of ADBs in Fe(0)/C-ADN
3.3. Influencing Characteristics of OC and DO on ADBs in Fe(0)/C-ADN
3.4. Quantification of 16S rRNA, nirS, and cbbL
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Li, J.; Wang, X.; Deng, S.-H.; Li, Z.; Zhang, B.; Li, D. Functional and Microbiological Responses of Iron–Carbon Galvanic Cell-Supported Autotrophic Denitrification to Organic Carbon Variation and Dissolved Oxygen Shaking. Water 2024, 16, 2455. https://doi.org/10.3390/w16172455
Li J, Wang X, Deng S-H, Li Z, Zhang B, Li D. Functional and Microbiological Responses of Iron–Carbon Galvanic Cell-Supported Autotrophic Denitrification to Organic Carbon Variation and Dissolved Oxygen Shaking. Water. 2024; 16(17):2455. https://doi.org/10.3390/w16172455
Chicago/Turabian StyleLi, Jinlong, Xiaowei Wang, Shi-Hai Deng, Zhaoxu Li, Bin Zhang, and Desheng Li. 2024. "Functional and Microbiological Responses of Iron–Carbon Galvanic Cell-Supported Autotrophic Denitrification to Organic Carbon Variation and Dissolved Oxygen Shaking" Water 16, no. 17: 2455. https://doi.org/10.3390/w16172455