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Article

Metagenomic Analysis of Bacterial Community Structure and Pollutant Removal Process in High-Altitude Municipal Wastewater Treatment Plants of Tibet, China

1
School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China
2
School of Ecology and Environment, Tibet University, Lhasa 850000, China
*
Author to whom correspondence should be addressed.
Water 2025, 17(9), 1284; https://doi.org/10.3390/w17091284
Submission received: 14 March 2025 / Revised: 19 April 2025 / Accepted: 23 April 2025 / Published: 25 April 2025
(This article belongs to the Section Wastewater Treatment and Reuse)

Abstract

The bacterial communities of activated sludge are correlative with influent characteristics, geographical discrepancies, and environmental variables, which are essential for wastewater treatment plants (WWTPs). However, the comprehensive deciphering of bacterial community diversity in high-altitude WWTPs is scarce in Tibet, China. This study collected activated sludge samples from four A2O WWTPs (2980–3650 m above sea level) in Tibet. Illumina NovaSeq high-throughput sequencing revealed that Proteobacteria was the most abundant phylum (46.2–62.9%), followed by Bacteroidetes (14.8–21.8%) and Actinobacteria (3.5–10.9%). Candidatus Accumulibacter (7.2–15.1%) was the dominant denitrifying polyphosphate-accumulati
Keywords: high-altitude; bacterial community; wastewater treatment plants; Illumina NovaSeq high-throughput sequencing; metabolism pathways high-altitude; bacterial community; wastewater treatment plants; Illumina NovaSeq high-throughput sequencing; metabolism pathways

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MDPI and ACS Style

Zhang, R.; Liu, Y.; Li, H.; Xiong, J.; Zhang, Q.; Li, P.; Liu, L.; Lu, X. Metagenomic Analysis of Bacterial Community Structure and Pollutant Removal Process in High-Altitude Municipal Wastewater Treatment Plants of Tibet, China. Water 2025, 17, 1284. https://doi.org/10.3390/w17091284

AMA Style

Zhang R, Liu Y, Li H, Xiong J, Zhang Q, Li P, Liu L, Lu X. Metagenomic Analysis of Bacterial Community Structure and Pollutant Removal Process in High-Altitude Municipal Wastewater Treatment Plants of Tibet, China. Water. 2025; 17(9):1284. https://doi.org/10.3390/w17091284

Chicago/Turabian Style

Zhang, Rui, Yiwen Liu, Haibo Li, Jian Xiong, Qiangying Zhang, Pengtao Li, Lingjie Liu, and Xuebin Lu. 2025. "Metagenomic Analysis of Bacterial Community Structure and Pollutant Removal Process in High-Altitude Municipal Wastewater Treatment Plants of Tibet, China" Water 17, no. 9: 1284. https://doi.org/10.3390/w17091284

APA Style

Zhang, R., Liu, Y., Li, H., Xiong, J., Zhang, Q., Li, P., Liu, L., & Lu, X. (2025). Metagenomic Analysis of Bacterial Community Structure and Pollutant Removal Process in High-Altitude Municipal Wastewater Treatment Plants of Tibet, China. Water, 17(9), 1284. https://doi.org/10.3390/w17091284

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