Bispecific CD3/HER2 Targeting FynomAb Induces Redirected T Cell-Mediated Cytolysis with High Potency and Enhanced Tumor Selectivity
Abstract
:1. Introduction
2. Results
2.1. CD3/HER2 Bispecific FynomAb COVA420 Potently Induces T Cell Mediated Cytotoxicity
2.2. COVA420 Shows IgG-Like Pharmacokinetic Profile and Inhibits SKOV-3 Tumor Growth In Vivo
2.3. COVA420 Selectively Kills Cells with High HER2 Expression, but Spares Cells with Low HER2 Expression
2.4. COVA420 Selectively Binds to Cells with High HER2 Expression
2.5. Discussion
3. Experimental Section
3.1. Protein Purification
3.2. Flow Cytometry, HER2 Quantification
3.3. In Vitro Redirected T Cell Cytotoxicity Assays
3.4. Pharmacokinetic Analysis
3.5. In Vivo Efficacy Studies
4. Conclusions
Supplementary Materials
Author Contributions
Conflicts of Interest
References
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Wuellner, U.; Klupsch, K.; Buller, F.; Attinger-Toller, I.; Santimaria, R.; Zbinden, I.; Henne, P.; Grabulovski, D.; Bertschinger, J.; Brack, S. Bispecific CD3/HER2 Targeting FynomAb Induces Redirected T Cell-Mediated Cytolysis with High Potency and Enhanced Tumor Selectivity. Antibodies 2015, 4, 426-440. https://doi.org/10.3390/antib4040426
Wuellner U, Klupsch K, Buller F, Attinger-Toller I, Santimaria R, Zbinden I, Henne P, Grabulovski D, Bertschinger J, Brack S. Bispecific CD3/HER2 Targeting FynomAb Induces Redirected T Cell-Mediated Cytolysis with High Potency and Enhanced Tumor Selectivity. Antibodies. 2015; 4(4):426-440. https://doi.org/10.3390/antib4040426
Chicago/Turabian StyleWuellner, Ulrich, Kristina Klupsch, Fabian Buller, Isabella Attinger-Toller, Roger Santimaria, Irene Zbinden, Patricia Henne, Dragan Grabulovski, Julian Bertschinger, and Simon Brack. 2015. "Bispecific CD3/HER2 Targeting FynomAb Induces Redirected T Cell-Mediated Cytolysis with High Potency and Enhanced Tumor Selectivity" Antibodies 4, no. 4: 426-440. https://doi.org/10.3390/antib4040426
APA StyleWuellner, U., Klupsch, K., Buller, F., Attinger-Toller, I., Santimaria, R., Zbinden, I., Henne, P., Grabulovski, D., Bertschinger, J., & Brack, S. (2015). Bispecific CD3/HER2 Targeting FynomAb Induces Redirected T Cell-Mediated Cytolysis with High Potency and Enhanced Tumor Selectivity. Antibodies, 4(4), 426-440. https://doi.org/10.3390/antib4040426