Shewanella oneidensis arcA Mutation Impairs Aerobic Growth Mainly by Compromising Translation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains, Plasmids, and Culture Conditions
2.2. Gene Knock In and Expression
2.3. Droplet Assays
2.4. Microscopic Analysis
2.5. Analysis of Gene Expression
2.6. Purification of Ribosomes
2.7. Electrophoretic Motility Shift Assay (EMSA)
2.8. Other Analyses
3. Results
3.1. The arcA Mutation Impairs Translation Efficacy
3.2. Reduced Ribosome Biosynthesis May Underlie Translation and Growth Defects of the S. oneidensis arcA Mutant
3.3. ArcA (but Not Fnr or Crp) Impacts Translation Efficiency
3.4. The ArcA Loss Reduces Ribosome Biosynthesis but Does Not Affect Ribosome Assembly
3.5. rpsA Is under the Direct Control of ArcA
3.6. Reduced Expression of rpsA Is, at Least in Part, Accountable for Defects in Aerobic Growth but Not in the Cell Envelope Integrity Caused by the ArcA Loss
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain or Plasmid | Description | Reference or Source |
---|---|---|
E. coli strains | ||
DH5α | Host for cloning | Lab stock |
WM3064 | ΔdapA, donor strain for conjugation | W. Metcalf, UIUC |
S. oneidensis strains | ||
MR-1 | Wild type | ATCC 700550 |
HG0624 | ∆crp derived from MR-1 | [15] |
HG2356 | ∆fnr derived from MR-1 | [15] |
HG3988 | ∆arcA derived from MR-1 | [24] |
HG3927W | eGFP knock-in at rplI derived from MR-1 | This study |
HG3927A | eGFP knock-in at rplI derived from ∆arcA | This study |
Plasmid | ||
pHGM01 | Apr Gmr Cmr suicide vector | [31] |
pHGEI01 | Kmr, integrative lacZ reporter vector | [32] |
pHGEN–Ptac | Kmr, IPTG-inducible expression vector | [33] |
pBBR–Cre | Spr, helper plasmid for antibiotic cassette removal | [18] |
pHG101–ArcA | Complementation vector carrying arcA | [29] |
pHGEI01–PaprE | PaprE–lacZ fusion within pHGEI01 | [29] |
pHGEI01–Psap | Psap–lacZ fusion within pHGEI01 | This study |
pHGEI01–PdtpA | PdtpA–lacZ fusion within pHGEI01 | This study |
pHGEI01–PdtpB | PdtpB–lacZ fusion within pHGEI01 | This study |
pHGEI01–PSO1505 | PSO1505–lacZ fusion within pHGEI01 | This study |
pHGEI01–PSO3195 | PSO3195–lacZ fusion within pHGEI01 | This study |
pHGEI01–PrplK | PrplK–lacZ fusion within pHGEI01 | This study |
pHGEI01–PsspA | PsspA–lacZ fusion within pHGEI01 | This study |
pHGEI01–PSO0783 | PSO0783–lacZ fusion within pHGEI01 | This study |
pHGEI01–PbtuD | PbtuD–lacZ fusion within pHGEI01 | This study |
pHGEI01–PnuoA | PnuoA–lacZ fusion within pHGEI01 | This study |
pHGEI01–PSO2061 | PSO2016–lacZ fusion within pHGEI01 | This study |
pHGEI01–PSO3282 | PSO3282–lacZ fusion within pHGEI01 | This study |
pHGEI01–PSO4542 | PSO4542–lacZ fusion within pHGEI01 | This study |
pHGEI01–PpspF | PpspF–lacZ fusion within pHGEI01 | This study |
pHGEI01–PhoxK | PhoxK–lacZ fusion within pHGEI01 | This study |
pHGEI01–PcsgB | PcsgB–lacZ fusion within pHGEI01 | This study |
pHGEI01–PrpsA | PrpsA–lacZ fusion within pHGEI01 | This study |
pHGEI01–PrpmB | PrpmB–lacZ fusion within pHGEI01 | This study |
pHGEN-Ptac-rpsA | Ptac-rpsA within pHGE-Ptac | This study |
pHGEN-Ptac-rpmB | Ptac-rpmB within pHGE-Ptac | This study |
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Xie, P.; Wang, J.; Liang, H.; Gao, H. Shewanella oneidensis arcA Mutation Impairs Aerobic Growth Mainly by Compromising Translation. Life 2021, 11, 926. https://doi.org/10.3390/life11090926
Xie P, Wang J, Liang H, Gao H. Shewanella oneidensis arcA Mutation Impairs Aerobic Growth Mainly by Compromising Translation. Life. 2021; 11(9):926. https://doi.org/10.3390/life11090926
Chicago/Turabian StyleXie, Peilu, Jiahao Wang, Huihui Liang, and Haichun Gao. 2021. "Shewanella oneidensis arcA Mutation Impairs Aerobic Growth Mainly by Compromising Translation" Life 11, no. 9: 926. https://doi.org/10.3390/life11090926