Simulated Microgravity Promotes Horizontal Gene Transfer of Antimicrobial Resistance Genes between Bacterial Genera in the Absence of Antibiotic Selective Pressure
Abstract
:1. Introduction
2. Methods
2.1. Co-Culture Experiments
2.2. Isolation of Colonies after HARV Growth
2.3. Designing Primers
2.4. PCR
- blaOXA-500_F (5′-CCGAGTTGTTCCAATCCCTTAT-3′) and
- blaOXA-500_R (5′-ATATGTTCCCGCCTCTACCT-3′) to amplify blaOXA-500
- and ISAba1_F (5′-ATGCAGCGCTTCTTTGCAGG-3′) and
- ISAba1_R (5′-AATGATTGGTGACAATGAAG-3′) to amplify ISAba1.
2.5. Calculating the Efficiency of Gene Transfer
2.6. Chromosome vs. Plasmid Location of blaOXA-500
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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S. aureus Recipient Strain | SMG (Range) | 1G (Range) |
---|---|---|
IF4SW | 4.21 × 10−7 (1.1 × 10−6 to 5.2 × 10−8) | 2.05 × 10−9 (0.0 to 4.1 × 10−9) |
IIF6SW | 4.54 × 10−8 (7.5 × 10−8 to 6.3 × 10−9) | 0.0 (no transfer) |
IF7SW | 3.20 × 10−7 (7.2 × 10−7 to 9.9 × 10−8) | 4.33 × 10−9 (7.5 × 10−9 to 2.2 × 10−9) |
IIF8SW | 4.38 × 10−7 (6.8 × 10−7 to 3.1 × 10−9) | 6.07 × 10−9 (0.0 to 1.2 × 10−9) |
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Urbaniak, C.; Grams, T.; Mason, C.E.; Venkateswaran, K. Simulated Microgravity Promotes Horizontal Gene Transfer of Antimicrobial Resistance Genes between Bacterial Genera in the Absence of Antibiotic Selective Pressure. Life 2021, 11, 960. https://doi.org/10.3390/life11090960
Urbaniak C, Grams T, Mason CE, Venkateswaran K. Simulated Microgravity Promotes Horizontal Gene Transfer of Antimicrobial Resistance Genes between Bacterial Genera in the Absence of Antibiotic Selective Pressure. Life. 2021; 11(9):960. https://doi.org/10.3390/life11090960
Chicago/Turabian StyleUrbaniak, Camilla, Tristan Grams, Christopher E. Mason, and Kasthuri Venkateswaran. 2021. "Simulated Microgravity Promotes Horizontal Gene Transfer of Antimicrobial Resistance Genes between Bacterial Genera in the Absence of Antibiotic Selective Pressure" Life 11, no. 9: 960. https://doi.org/10.3390/life11090960