Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis
Abstract
:1. Introduction
2. Materials and Methods
2.1. BA Isolates
2.2. Bacterial Culture Preparation and Inactivation Procedure
2.3. PCR
2.4. Genome Sequencing, Assembly, and Annotation of BA Isolates
2.5. LC-MS/MS Analysis of BA Isolates
2.6. Plasmid Detection with ABRicate
2.7. Data Processing and Bioinformatics Analysis
2.8. Identification of Species-Specific Peptides
3. Results
3.1. Cluster Analyses
3.2. Analysis of Plasmid Content
3.3. Identification of Species-Specific Candidate Marker Peptides for BA
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peptide Sequence | Prokka Annotation | Median Intensity Percentile Rank | Identified in LC-MS/MS Measurements (%) |
---|---|---|---|
QLSDVAEEDVNR | Short-chain-enoyl-CoA hydratase | 91.20 | 100.00 |
EINGQAQTQTTVTETK | putative peptidoglycan endopeptidase LytE | 52.24 | 100.00 |
FQMGNQHYSIDVHK | hypothetical protein | 85.65 | 97.06 |
FFETHDYK | Spore germination protein YaaH | 84.96 | 86.27 |
TPGHGIYTGIK | Asparagine synthetase glutamine-hydrolyzing 3 | 73.83 | 83.33 |
NIAQGAALATDTK | p-aminobenzoyl-glutamate hydrolase subunit B | 59.47 | 81.37 |
TLDNAVEELTQITGQK | 50S ribosomal protein L5 | 62.58 | 81.37 |
EDSPLVTLTGK | PhoH-like protein | 63.61 | 75.49 |
NSSHWAEEDTILSGVK | N-acetyltransferase YodP | 64.46 | 73.53 |
AQASGASIQSTNASYGTEFATETDVHAVK | Small, acid-soluble spore protein gamma-type | 92.41 | 66.67 |
VLTSGQEAVSHNFLGDK | putative 3-hydroxyacyl-CoA dehydrogenase | 61.27 | 55.88 |
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Witt, N.; Galante, D.; Andreotti, S.; Abdel Glil, M.; Fasanella, A.; Meierhofer, D.; Tomaso, H. Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis. Life 2022, 12, 1549. https://doi.org/10.3390/life12101549
Witt N, Galante D, Andreotti S, Abdel Glil M, Fasanella A, Meierhofer D, Tomaso H. Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis. Life. 2022; 12(10):1549. https://doi.org/10.3390/life12101549
Chicago/Turabian StyleWitt, Natalie, Domenico Galante, Sandro Andreotti, Mostafa Abdel Glil, Antonio Fasanella, David Meierhofer, and Herbert Tomaso. 2022. "Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis" Life 12, no. 10: 1549. https://doi.org/10.3390/life12101549