The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Transgenic Mice
2.2. Genotyping
2.3. OTM Model
2.4. MicroCT
2.5. Histology and TRAP Stain
2.6. Immunofluorescence Stain
2.7. Real-Time PCR
2.8. Western Blot
2.9. Statistics
3. Results
3.1. IFT80 Expression and Cilia Number Significantly Decreases in Osteocytes of DMP1 CRE+.IFT80f/f Mice
3.2. IFT80 Deletion in Osteocytes Does Not Affect Orthodontic Tooth Movement
3.3. Deletion of IFT80 in Osteocytes Does Not Affect Bone Remodeling during OTM and Physiologic Bone Remodeling
3.4. Loss of IFT80 in Osteocytes Slightly Affects Osteoclast Formation during OTM but without Statistical Difference
3.5. Ablation of IFT80 in Osteocytes Does Not Alter Expression Levels of Sclerostin and RANKL
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jeon, H.H.; Kang, J.; Li, J.; Kim, D.; Yuan, G.; Almer, N.; Liu, M.; Yang, S. The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study. Life 2022, 12, 1147. https://doi.org/10.3390/life12081147
Jeon HH, Kang J, Li J, Kim D, Yuan G, Almer N, Liu M, Yang S. The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study. Life. 2022; 12(8):1147. https://doi.org/10.3390/life12081147
Chicago/Turabian StyleJeon, Hyeran Helen, Jessica Kang, Jiahui (Madelaine) Li, Douglas Kim, Gongsheng Yuan, Nicolette Almer, Min Liu, and Shuying Yang. 2022. "The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study" Life 12, no. 8: 1147. https://doi.org/10.3390/life12081147