The Role of V-Set Ig Domain-Containing 4 in Chronic Kidney Disease Models
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Studies
2.2. Podocyte Culture
2.3. Quantitative Real-Time Polymerase Chain Reactions
2.4. Immunohistochemical Staining for VSIG4
2.5. Western Blot Analysis
2.6. Statistical Analysis
3. Results
3.1. UUO Model
3.2. Doxorubicin-Induced Kidney Injury Model
3.2.1. Baseline Characteristics
3.2.2. VSIG4 Expression
3.3. Doxorubicin-Induced Podocyte Injury Model
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Yang, Y.; Xu, G. Update on pathogenesis of glomerular hyperfiltration in early diabetic kidney disease. Front. Endocrinol. 2022, 13, 872918. [Google Scholar] [CrossRef] [PubMed]
- Couser, W.; Johnson, R. The etiology of glomerulonephritis: Roles of infection and autoimmunity. Kidney Int. 2014, 86, 905–914. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Voelker, J.; Berg, P.; Sheetz, M.; Duffin, K.; Shen, T.; Moser, B.; Greene, T.; Blumenthal, S.; Rychlik, I.; Yagil, Y.; et al. Anti-TGF-β1 antibody therapy in patients with diabetic nephropathy. J. Am. Soc. Nephrol. 2017, 28, 953–962. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Adler, S.; Schwartz, S.; Williams, M.; Arauz-Pacheco, C.; Bolton, W.; Lee, T.; Li, D.; Neff, T.; Urquilla, P.; Sewell, K. Phase 1 study of anti-CTGF monoclonal antibody in patients with diabetes and microalbuminuria. Clin. J. Am. Soc. Nephrol. 2010, 5, 1420–1428. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Vogt, L.; Schmitz, N.; Kurrer, M.; Bauer, M.; Hinton, H.; Behnke, S.; Gatto, D.; Sebbel, P.; Beerli, R.; Sonderegger, I.; et al. VSIG4, a B7 family-related protein, is a negative regulator of T cell activation. J. Clin. Investig. 2006, 116, 2817–2826. [Google Scholar] [CrossRef] [PubMed]
- Jung, K.; Seo, S.; Choi, I. Endogenous VSIG4 negatively regulates the helper T cell-mediated antibody response. Immunol. Lett. 2015, 165, 78–83. [Google Scholar] [CrossRef] [PubMed]
- Liao, Y.; Guo, S.; Chen, Y.; Cao, D.; Xu, H.; Yang, C.; Fei, L.; Ni, B.; Ruan, Z. VSIG4 expression on macrophages facilitates lung cancer development. Lab. Investig. 2014, 94, 706–715. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhang, X.; Qian, Y.; Li, Z.; Zhang, N.; Xie, Y. Let-7g-5p inhibits epithelial-mesenchymal transition consistent with reduction of glioma stem cell phenotypes by targeting VSIG4 in glioblastoma. Oncol. Rep. 2016, 36, 2967–2975. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, Y.; Wang, Y.; Wang, D.; Hou, W.; Zhang, Y.; Li, M.; Li, F.; Mu, J.; Du, X.; Pang, F.; et al. Costimulatory molecule VSIG4 exclusively expressed on macrophages alleviates renal tubulointerstitial injury in VSIG4 KO mice. J. Nephrol. 2014, 27, 29–36. [Google Scholar] [CrossRef] [PubMed]
- Kim, S.; Oh, S.; Park, S.; Hur, D.; Hong, S.; Han, S. Epstein-Barr virus-encoded latent membrane protein 1 induces epithelial to mesenchymal transition by inducing V-set Ig domain containing 4 (VSIG4) expression via NF-kB in renal tubular epithelial HK-2 cells. Biochem. Biophys. Res. Commun. 2017, 492, 316–322. [Google Scholar] [CrossRef] [PubMed]
- Gong, E.; Jo, H.; Park, S.; Cha, D.; Hur, D.; Han, S. VSIG4 Induces epithelial-mesenchymal transition of renal tubular cells under high-glucose conditions. Life 2020, 10, 354. [Google Scholar] [CrossRef]
- Xing, Y.; Ding, J.; Fan, Q.; Guan, N. Diversities of podocyte molecular changes induced by different antiproteinuria drugs. Exp. Biol. Med. 2006, 231, 585–593. [Google Scholar] [CrossRef]
- Szalay, C.; Erdélyi, K.; Kökény, G.; Lajtár, E.; Godó, M.; Révész, C.; Kaucsár, T.; Kiss, N.; Sárközy, M.; Csont, T.; et al. Oxidative/nitrative stress and inflammation drive progression of doxorubicin-induced renal fibrosis in rats as revealed by comparing a normal and a fibrosis-resistant rat rtrain. PLoS ONE 2015, 10, e0127090. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Cha, J.; Kang, Y.; Hyun, Y.; Han, S.; Jee, Y.; Han, K.; Han, J.; Cha, D. Sulodexide improves renal function through reduction of vascular endothelial growth factor in type 2 diabetic rats. Life Sci. 2013, 92, 1118–1124. [Google Scholar] [CrossRef] [PubMed]
- Zeng, M.; Liu, J.J.; Yang, W.; Zhang, S.; Liu, F.; Dong, Z.; Peng, Y.; Sun, L.; Xiao, L. Identification of key biomarkers in diabetic nephropathy via bioinformatic analysis. J. Cell Biochem. 2019, 120, 8676–8688. [Google Scholar] [CrossRef] [PubMed]
- Xu, B.; Wang, L.; Zhan, H.; Zhao, L.; Wang, Y.; Shen, M.; Xu, K.; Li, L.; Luo, X.; Zhou, S.; et al. Investigation of the mechanism of complement system in diabetic nephropathy via bioinformatics analysis. J. Diabetes Res. 2021, 2021, 5546199. [Google Scholar] [CrossRef] [PubMed]
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Han, S.Y.; Ghee, J.Y.; Cha, J.J.; Kang, Y.S.; Kim, H.S.; Hur, D.Y.; Cha, D.R. The Role of V-Set Ig Domain-Containing 4 in Chronic Kidney Disease Models. Life 2023, 13, 277. https://doi.org/10.3390/life13020277
Han SY, Ghee JY, Cha JJ, Kang YS, Kim HS, Hur DY, Cha DR. The Role of V-Set Ig Domain-Containing 4 in Chronic Kidney Disease Models. Life. 2023; 13(2):277. https://doi.org/10.3390/life13020277
Chicago/Turabian StyleHan, Sang Youb, Jung Yeon Ghee, Jin Joo Cha, Young Sun Kang, Han Seong Kim, Dae Young Hur, and Dae Ryong Cha. 2023. "The Role of V-Set Ig Domain-Containing 4 in Chronic Kidney Disease Models" Life 13, no. 2: 277. https://doi.org/10.3390/life13020277