Arachidonic Acid Added during the Differentiation Phase of 3T3-L1 Cells Exerts Anti-Adipogenic Effect by Reducing the Effects of Pro-Adipogenic Prostaglandins
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Culture of 3T3-L1 Cells and Induction of Adipogenesis
2.3. Quantification of Intracellular TAGs and Cellular Proteins
2.4. Quantification of PGs by ELISA
2.5. Quantification of Gene Expression
2.6. Statistical Analyses
3. Results
3.1. Effects of AA or LA Added during Only the Differentiation Phase of 3T3-L1 Cells on MDI-Induced Adipogenesis
3.2. Types of PGs Biosynthesized by Adding AA during Only the Differentiation Phase of 3T3-L1 Cells and Effects of PGs Added during Only the Differentiation Phase on MDI-Induced Adipogenesis
3.3. Adipogenesis-Related Gene Expression in Response to AA during the Differentiation Phase
3.4. Effects of AA Coexistent with Pro-Adipogenic PGs during the Differentiation Phase of 3T3-L1 Cells on MDI-Induced Adipogenesis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Target Genes | Primers (5→3′) | Length (bp) | Tm (°C) | Product Length (bp) |
---|---|---|---|---|
C/Ebpβ | Fw: CGGGTTTCGGGACTTGAT | 18 | 56.97 | 93 |
Rv: GCCCGGCTGACAGTTACAC | 19 | 61.03 | ||
C/Ebpδ | Fw: GACTCCTGCCATGTACGACG | 20 | 60.53 | 118 |
Rv: GTTGAAGAGGTCGGCGAAGA | 20 | 60.04 | ||
Pparγ | Fw: CTTCGCTGATGCACTGCCTAT | 21 | 60.81 | 216 |
Rv: GGGTCAGCTCTTGTGAATGGA | 21 | 60.00 | ||
C/Ebpα | Fw: GCCAAGAAGTCGGTGGACA | 19 | 59.93 | 110 |
Rv: GTCTCCACGTTGCGTTGTTT | 20 | 59.62 | ||
Lpl | Fw: TTGCAGAGAGAGGACTCGGA | 20 | 59.96 | 125 |
Rv: GGAGTTGCACCTGTATGCCT | 20 | 60.04 | ||
Glut4 | Fw: GGATTCCATCCCACAAGGCA | 20 | 60.03 | 158 |
Rv: CCAACACGGCCAAGACATTG | 20 | 60.04 | ||
Leptin | Fw: TTTCACACACGCAGTCGGTA | 20 | 59.90 | 149 |
Rv: CACATTTTGGGAAGGCAGGC | 20 | 60.04 | ||
β-Actin | Fw: GCGGGCGACGATGCT | 15 | 59.84 | 197 |
Rv: TGCCAGATCTTCTCCATGTCG | 21 | 59.86 |
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Nartey, M.N.N.; Jisaka, M.; Syeda, P.K.; Nishimura, K.; Shimizu, H.; Yokota, K. Arachidonic Acid Added during the Differentiation Phase of 3T3-L1 Cells Exerts Anti-Adipogenic Effect by Reducing the Effects of Pro-Adipogenic Prostaglandins. Life 2023, 13, 367. https://doi.org/10.3390/life13020367
Nartey MNN, Jisaka M, Syeda PK, Nishimura K, Shimizu H, Yokota K. Arachidonic Acid Added during the Differentiation Phase of 3T3-L1 Cells Exerts Anti-Adipogenic Effect by Reducing the Effects of Pro-Adipogenic Prostaglandins. Life. 2023; 13(2):367. https://doi.org/10.3390/life13020367
Chicago/Turabian StyleNartey, Michael N. N., Mitsuo Jisaka, Pinky Karim Syeda, Kohji Nishimura, Hidehisa Shimizu, and Kazushige Yokota. 2023. "Arachidonic Acid Added during the Differentiation Phase of 3T3-L1 Cells Exerts Anti-Adipogenic Effect by Reducing the Effects of Pro-Adipogenic Prostaglandins" Life 13, no. 2: 367. https://doi.org/10.3390/life13020367