Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Lab Animals
2.2. Purification of Total RNA
2.3. Real-Time PCR
2.4. Statistical Analysis
3. Results
3.1. Laser Therapy Normalizes the Histological Features of Skin Damaged with Bleomycin
3.2. Analysis of Gene Expression Revealed Significant Changes in the Expression of Matrix Metalloproteinases
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Reference Sequence | Primer Name | Primer Sequence | Product Size, bp |
---|---|---|---|---|
MMP2 | NM_008610.3 | MMP2 forward | CCCTGATAACCTGGATGCCG | 214 |
MMP2 reverse | AGGCTGCTTCACATCCTTCAC | |||
MMP3 | NM_010809.2 | MMP3 forward | CATGAACTTGGCCACTCCCT | 179 |
MMP3 reverse | GTGGGTACCACGAGGACATC | |||
MMP9 | NM_013599.5 | MMP9 forward | TGGTCTTCCCCAAAGACCTG | 216 |
MMP9 reverse | CACAGCGTGGTGTTCGAATG | |||
18S RNA | NR_003278.3 | 18S RNA forward | GCAATTATTCCCCATGAACG | 123 |
18S RNA reverse | TTGGATGGTTTAGTGAGGCC |
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Soboleva, A.G.; Sobolev, V.V.; Karapetyan, M.M.; Mezentsev, A.; Rud’ko, O.I.; Davydova, E.D.; Mogulevtseva, J.A.; Zhukova, O.V.; Korsunskaya, I.M. Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis. Life 2023, 13, 810. https://doi.org/10.3390/life13030810
Soboleva AG, Sobolev VV, Karapetyan MM, Mezentsev A, Rud’ko OI, Davydova ED, Mogulevtseva JA, Zhukova OV, Korsunskaya IM. Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis. Life. 2023; 13(3):810. https://doi.org/10.3390/life13030810
Chicago/Turabian StyleSoboleva, Anna G., Vladimir V. Sobolev, Mari M. Karapetyan, Alexandre Mezentsev, Olga I. Rud’ko, Evgenia D. Davydova, Julia A. Mogulevtseva, Olga V. Zhukova, and Irina M. Korsunskaya. 2023. "Laser Therapy Changes the Expression of Matrix Metalloproteinases in Bleomycin-Induced Skin Fibrosis" Life 13, no. 3: 810. https://doi.org/10.3390/life13030810