Ocimum sanctum Alters the Lipid Landscape of the Brain Cortex and Plasma to Ameliorate the Effect of Photothrombotic Stroke in a Mouse Model
Abstract
:1. Introduction
2. Material and Methods
2.1. Animals
2.2. Experimental Groups
2.3. Photothrombotic Lesion (PTL)
2.4. Cytochrome Oxidase Reaction
2.5. Sample Collection and Preparation
2.6. Mass Spectrometry
Mass Spectrometry for Metabolomics of Tulsi Extract
2.7. Software Analysis
2.8. Statistical Analysis
3. Results
3.1. Tulsi Modulates Lipidomic Signature in the Lesioned Cortical Hemisphere of Mice with Photothrombotic Ischemic Stroke-like Lesion
3.2. Comparison of Lipidome in Photothrombotic Ischemia-Induced Brain of Mice Treated with Tulsi vs. Ibuprofen
3.3. Effect of Tulsi in Modulating Lipidome Signature of Plasma in Mice with Photothrombotic Ischemic Lesion of the Cerebral Cortex
3.4. Integration of Brain and Plasma Lipidomic Analysis
3.5. Untargeted Lipidomic and Metabolomics of Ocimum sanctum
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Yadav, I.; Sharma, N.; Velayudhan, R.; Fatima, Z.; Maras, J.S. Ocimum sanctum Alters the Lipid Landscape of the Brain Cortex and Plasma to Ameliorate the Effect of Photothrombotic Stroke in a Mouse Model. Life 2023, 13, 1877. https://doi.org/10.3390/life13091877
Yadav I, Sharma N, Velayudhan R, Fatima Z, Maras JS. Ocimum sanctum Alters the Lipid Landscape of the Brain Cortex and Plasma to Ameliorate the Effect of Photothrombotic Stroke in a Mouse Model. Life. 2023; 13(9):1877. https://doi.org/10.3390/life13091877
Chicago/Turabian StyleYadav, Inderjeet, Nupur Sharma, Rema Velayudhan, Zeeshan Fatima, and Jaswinder Singh Maras. 2023. "Ocimum sanctum Alters the Lipid Landscape of the Brain Cortex and Plasma to Ameliorate the Effect of Photothrombotic Stroke in a Mouse Model" Life 13, no. 9: 1877. https://doi.org/10.3390/life13091877