The Complete Mitochondrial Genome Sequence of Eimeria kongi (Apicomplexa: Coccidia)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Parasites
2.2. Genomic DNA Extraction and PCR Amplification
2.2.1. Oocyst Purification
2.2.2. Genomic DNA Extraction
2.2.3. PCR Amplification
2.3. Genome Annotation and Sequence Analysis
2.4. Clustal Alignment and Phylogenetic Analyses
3. Results and Discussion
3.1. Analyzing the mtDNA Composition of E. kongi
3.2. Clustal Alignment and Phylogenetic Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Species | Accession Number | Species | Accession Number |
---|---|---|---|
E. kongi | NC_072899 | E. gallopavonis | KJ608413 |
E. vejdovskyi | KP025692 | E. meleagrimitis | KJ608414 |
E. media | KP025691 | E. necatrix | KX094954 |
E. magna | KF419217 | E. tenella | HQ702484 |
E. intestinalis | KP009592 | E. acervulina | HQ702479 |
E. flavescens | KP025693 | E. praecox | HQ702483 |
E. irresidua | KP025690 | E. maxima | HQ702481 |
E. dispersa | KJ608416 | E. brunetti | HQ702480 |
E. adenoides | KJ608415 | E. mitis | KF501573 |
E. meleagridis | KJ608418 |
Genes | Nucleotide No. | Size (bp) | Start Codon | Stop Codon |
---|---|---|---|---|
SSU4 | 1–82 | 82 | ||
cytb | 128–1207 | 1080 | ATG | TAA |
cox1 | 1240–2682 | 1443 | ATG | TAA |
LSU11 | 2722–2849 | 128 | ||
LSU12 | 2851–2949 | 99 | ||
LSU4 | 3090–3114 | 24 | ||
SSU12 | 3244–3304 | 61 | ||
LSU13 | 3305–3379 | 75 | ||
LSU5 | 3403–3451 | 49 | ||
SSU10 | 3460–3524 | 65 | ||
SSU8 | 3538–3596 | 59 | ||
LSU10 | 3637–3669 | 33 | ||
LSU15 | 3678–3733 | 56 | ||
LSU8 | 3757–3837 | 81 | ||
SSU5 | 3883–3974 | 92 | ||
LSU2 | 3992–4057 | 66 | ||
LSU1 | 4083–4261 | 179 | ||
cox3 | 4279–5034 | 756 | TTA | TAA |
SSU1 | 5044–5073 | 30 | ||
LSU6 | 5102–5195 | 94 | ||
LSU3 | 5272–5295 | 24 | ||
LSU7 | 5331–5403 | 73 | ||
SSU6 | 5467–5582 | 116 | ||
LSU9 | 5684–5871 | 188 | ||
SSU11 | 5970–6006 | 37 | ||
SSU9 | 6032–6212 | 181 |
Species | A (%) | T (%) | G (%) | C (%) | Total |
---|---|---|---|---|---|
E. mitis | 30.9 | 36.5 | 16.3 | 16.3 | 6408 |
E. irresidua | 29.8 | 35.6 | 17 | 17.6 | 6259 |
E. kongi | 29.8 | 35.6 | 17 | 17.6 | 6258 |
E. flavescens | 30 | 35.5 | 16.9 | 17.6 | 6258 |
E. magna | 29.7 | 35.4 | 17.1 | 17.7 | 6249 |
E. intestinalis | 29.7 | 35.5 | 17.1 | 17.7 | 6247 |
E. media | 29.6 | 35.5 | 17.1 | 17.7 | 6245 |
E. vejdovskyi | 29.6 | 35.5 | 17.2 | 17.7 | 6240 |
E. dispersa | 29.9 | 35.6 | 16.8 | 17.6 | 6238 |
E. gallopavonis | 30.1 | 34.8 | 16.9 | 18.3 | 6215 |
E. tenella | 29.8 | 35.2 | 16.9 | 18.1 | 6213 |
E. meleagridis | 29.8 | 34.9 | 17 | 18.3 | 6212 |
E. necatrix | 29.8 | 35.2 | 16.9 | 18.2 | 6212 |
E. adenoides | 29.8 | 34.8 | 17 | 18.4 | 6211 |
E. acervulina | 30.1 | 35.3 | 17 | 17.6 | 6179 |
E. praecox | 30.1 | 35.5 | 17.1 | 17.3 | 6172 |
E. maxima | 30 | 34.5 | 17.5 | 17.9 | 6167 |
E. meleagrimitis | 30 | 33.5 | 17.3 | 19.2 | 6165 |
E. brunetti | 29.8 | 35.8 | 17.1 | 17.4 | 6156 |
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Shi, Y.; Fang, S.; Gu, X.; Hao, C.; Du, F.; Cui, P.; Tang, X. The Complete Mitochondrial Genome Sequence of Eimeria kongi (Apicomplexa: Coccidia). Life 2024, 14, 699. https://doi.org/10.3390/life14060699
Shi Y, Fang S, Gu X, Hao C, Du F, Cui P, Tang X. The Complete Mitochondrial Genome Sequence of Eimeria kongi (Apicomplexa: Coccidia). Life. 2024; 14(6):699. https://doi.org/10.3390/life14060699
Chicago/Turabian StyleShi, Yubo, Sufang Fang, Xiaolong Gu, Chengyu Hao, Fangchen Du, Ping Cui, and Xinming Tang. 2024. "The Complete Mitochondrial Genome Sequence of Eimeria kongi (Apicomplexa: Coccidia)" Life 14, no. 6: 699. https://doi.org/10.3390/life14060699