This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessArticle
Comparative Analysis of Hulless Barley Transcriptomes to Regulatory Effects of Phosphorous Deficiency
by
Likun An
Likun An 1,2,3,4,†,
Ziao Wang
Ziao Wang 1,2,3,4,†,
Yongmei Cui
Yongmei Cui 1,2,3,4,
Yixiong Bai
Yixiong Bai 1,2,3,4
,
Youhua Yao
Youhua Yao 1,2,3,4
,
Xiaohua Yao
Xiaohua Yao 1,2,3,4 and
Kunlun Wu
Kunlun Wu 1,2,3,4,*
1
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China
2
Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources, Xining 810016, China
3
Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining 810016, China
4
Qinghai Subcenter of National Hulless Barley Improvement, Xining 810016, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Life 2024, 14(7), 904; https://doi.org/10.3390/life14070904 (registering DOI)
Submission received: 22 June 2024
/
Revised: 11 July 2024
/
Accepted: 15 July 2024
/
Published: 19 July 2024
Abstract
Hulless barley is a cold-resistant crop widely planted in the northwest plateau of China. It is also the main food crop in this region. Phosphorus (P), as one of the important essential nutrient elements, regulates plant growth and defense. This study aimed to analyze the development and related molecular mechanisms of hulless barley under P deficiency and explore the regulatory genes so as to provide a basis for subsequent molecular breeding research. Transcriptome analysis was performed on the root and leaf samples of hulless barley cultured with different concentrations of KH2PO4 (1 mM and 10 μM) Hoagland solution. A total of 46,439 genes were finally obtained by the combined analysis of leaf and root samples. Among them, 325 and 453 genes had more than twofold differences in expression. These differentially expressed genes (DEGs) mainly participated in the abiotic stress biosynthetic process through Gene Ontology prediction. Moreover, the Kyoto Encyclopedia of Genes and Genomes showed that DEGs were mainly involved in photosynthesis, plant hormone signal transduction, glycolysis, phenylpropanoid biosynthesis, and synthesis of metabolites. These pathways also appeared in other abiotic stresses. Plants initiated multiple hormone synergistic regulatory mechanisms to maintain growth under P-deficient conditions. Transcription factors (TFs) also proved these predictions. The enrichment of ARR-B TFs, which positively regulated the phosphorelay-mediated cytokinin signal transduction, and some other TFs (AP2, GRAS, and ARF) was related to plant hormone regulation. Some DEGs showed different values in their FPKM (fragment per kilobase of transcript per million mapped reads), but the expression trends of genes responding to stress and phosphorylation remained highly consistent. Therefore, in the case of P deficiency, the first response of plants was the expression of stress-related genes. The effects of this stress on plant metabolites need to be further studied to improve the relevant regulatory mechanisms so as to further understand the importance of P in the development and stress resistance of hulless barley.
Share and Cite
MDPI and ACS Style
An, L.; Wang, Z.; Cui, Y.; Bai, Y.; Yao, Y.; Yao, X.; Wu, K.
Comparative Analysis of Hulless Barley Transcriptomes to Regulatory Effects of Phosphorous Deficiency. Life 2024, 14, 904.
https://doi.org/10.3390/life14070904
AMA Style
An L, Wang Z, Cui Y, Bai Y, Yao Y, Yao X, Wu K.
Comparative Analysis of Hulless Barley Transcriptomes to Regulatory Effects of Phosphorous Deficiency. Life. 2024; 14(7):904.
https://doi.org/10.3390/life14070904
Chicago/Turabian Style
An, Likun, Ziao Wang, Yongmei Cui, Yixiong Bai, Youhua Yao, Xiaohua Yao, and Kunlun Wu.
2024. "Comparative Analysis of Hulless Barley Transcriptomes to Regulatory Effects of Phosphorous Deficiency" Life 14, no. 7: 904.
https://doi.org/10.3390/life14070904
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article Access Statistics
For more information on the journal statistics, click
here.
Multiple requests from the same IP address are counted as one view.