Development of the PD9-9 Monoclonal Antibody for Identifying Porcine Bone Marrow-Derived Dendritic Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cells
2.3. Hybridoma Technology
2.4. Flow Cytometry
2.5. Immunostaining
2.6. Cell Proliferation Assay
3. Results
3.1. Production of mAbs against Porcine DCs
3.2. mAb PD9-9 Reactivity during DC Differentiation
3.3. Distinguishing DCs from Macrophages
3.4. Effects on DC Proliferation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Clone | Heavy Chain | Light Chain |
---|---|---|
PD9-7 | IgG1 | ϰ |
PD9-9 | IgG1 | ϰ |
PD10-3 | IgG1 | ϰ |
G7 | IgG1 | ϰ |
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Kim, S.E.; Kim, Y.K.; Oh, K.B.; Hwang, J.H. Development of the PD9-9 Monoclonal Antibody for Identifying Porcine Bone Marrow-Derived Dendritic Cells. Life 2024, 14, 1054. https://doi.org/10.3390/life14091054
Kim SE, Kim YK, Oh KB, Hwang JH. Development of the PD9-9 Monoclonal Antibody for Identifying Porcine Bone Marrow-Derived Dendritic Cells. Life. 2024; 14(9):1054. https://doi.org/10.3390/life14091054
Chicago/Turabian StyleKim, Sang Eun, Young Kyu Kim, Keon Bong Oh, and Jeong Ho Hwang. 2024. "Development of the PD9-9 Monoclonal Antibody for Identifying Porcine Bone Marrow-Derived Dendritic Cells" Life 14, no. 9: 1054. https://doi.org/10.3390/life14091054