The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye
Abstract
:1. Introduction
2. Material and Method
2.1. Acquisition of Samples
2.2. Bacterial Culture
2.3. C. Pseudotuberculosis-Specific PCR
2.4. 16S rRNA Gene Sequencing and Phylogenetic Analysis
2.5. Antibiotic Sensitivity Test
3. Results
3.1. Bacterial Culture Findings
3.2. C. Pseudotuberculosis-Specific PCR Findings
3.3. 16S rRNA Gene Sequencing and Phylogenetic Analysis Findings
3.4. Antibiotic Sensitivity Test Findings
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Species | Primer Sequence (5′—3′) | Target Gene | PCR Component (25 μL) | Thermal Cycle | Amplicon (bp) | Reference |
---|---|---|---|---|---|---|
C. pseudotuberculosis | PLDF- ATAAGCGTAAGCAGGGAGCA PLDR1- ATCAGCGGTGATTGTCTTCC PLDR2- ATCAGCGGTGATTGTCTTCCAGG | pld |
| One cycle:
| 203 | [13] |
Unknown species specific | 27F- AGAGTTTGATC(AC)TGGCTCAG 1492R- ACGG(CT)TTACCTTGTTACGACTT | 16S rRNA | One cycle:
| 1465 | [16] |
Number of Isolate | Cultural and Phenotypic Characteristics | Species Name | NCBI Accession Number |
---|---|---|---|
1 | Gram-negative, bacilli | P. helleri | OR512107 |
2 | Gram-negative, bacilli | P. synxantha | OR512109 |
3 | Gram-negative, bacilli | P. moraviensis | OR512111 |
5 | Gram-negative, bacilli | P. libanensis | OR512104 |
6 | Gram-negative, bacilli | P. fluorescens | OR512105 |
7 | Gram-negative, bacilli | P. fluorescens | OR512106 |
8 | Gram-negative, bacilli | P. azotoformans | OR512112 |
9 | Gram-negative, bacilli | P. gessardii | OR512108 |
10 | Gram-negative, cocobacilli | E. cancerogenus | OR512110 |
Antibiotic (Concentration) | Susceptible | Intermediate | Resistant |
---|---|---|---|
KF (30 µg) | 0 (0%) | 0 (0%) | 30 (100%) |
EFT (30 µg) | 0 (0%) | 0 (0%) | 30 (100%) |
OBS (5 µg) | 0 (0%) | 0 (0%) | 30 (100%) |
CFP (75 µg) | 27 (90%) | 0 (0%) | 3 (10%) |
AML (25 µg) | 0 (0%) | 0 (0%) | 30 (100%) |
LS (109 µg) | 27 (90%) | 0 (0%) | 3 (10%) |
PNV (40 µg) | 18 (60%) | 0 (0%) | 12 (40%) |
S (10 U) | 0 (0%) | 0 (0%) | 30 (100%) |
BCDD (10 U) | 0 (0%) | 0 (0%) | 30 (100%) |
Antibiotic (Concentration) | Susceptible | Intermediate | Resistant |
---|---|---|---|
KF (30 µg) | 0 (0%) | 0 (0%) | 8 (100%) |
EFT (30 µg) | 0 (100%) | 0 (0%) | 8 (100%) |
OBS (5 µg) | 0 (0%) | 0 (0%) | 8 (100%) |
CFP (75 µg) | 8 (100%) | 0 (0%) | 0 (0%) |
AML (25 µg) | 0 (100%) | 1 (11%) | 8 (89%) |
LS (109 µg) | 7 (87.5%) | 0 (0%) | 1 (12.5%) |
PNV (40 µg) | 0 (0%) | 0 (0%) | 8 (0%) |
S (10 U) | 1 (12.5%) | 4 (50%) | 3 (37.5%) |
BCDD (10 U) | 0 (0%) | 0 (0%) | 8 (100%) |
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Tarhane, S.; Büyük, F. The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye. Life 2024, 14, 1078. https://doi.org/10.3390/life14091078
Tarhane S, Büyük F. The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye. Life. 2024; 14(9):1078. https://doi.org/10.3390/life14091078
Chicago/Turabian StyleTarhane, Serdal, and Fatih Büyük. 2024. "The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye" Life 14, no. 9: 1078. https://doi.org/10.3390/life14091078